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Diazomycin B

Product Name
Diazomycin B
CAS No.
7644-67-9
Chemical Name
Diazomycin B
Synonyms
NSC-56654;Azotomycin;DuazomycinB;Diazomycin B;Antibiotic-1719;Azotomycin sodium salt;Diazomycin B USP/EP/BP;Antibiotic produced by streptomyces ambofaciens;6-Diazo-N-(6-diazo-N-L-γ-glutamyl-5-oxo-L-norleucyl)-5-oxo-L-norleucine
CBNumber
CB1875681
Molecular Formula
C17H23N7O8
Formula Weight
453.41
MOL File
7644-67-9.mol
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Diazomycin B Property

Boiling point:
560.56°C (rough estimate)
Density 
1.2516 (rough estimate)
refractive index 
1.6800 (estimate)
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Safety

Toxicity
TDLo ivn-hmn: 192 mg/kg/4D:GIT CTRRDO 61,1719,77
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

American Custom Chemicals Corporation
Product number
API0013451
Product name
AZOTOMYCIN
Purity
95.00%
Packaging
5MG
Price
$504.69
Updated
2021/12/16
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Diazomycin B Chemical Properties,Usage,Production

Originator

Azotomycin,ZYF Pharm

Uses

Antineoplastic.

Manufacturing Process

Azotomycin is anticancer antibiotic produced by Streptomyces ambofaciens. Total sythesis of it from γ-benzyl-N-tert-butyloxycarbonyl-L-glutamic acid (γ- OBzl-N-Boc-L-Glu) has been accomplished in nine steps. The mixed carbonic anhydride method was chosen for peptide bond formation. Commerically available γ-OBzl-N-Boc-L-Glu was esterified with ethereal diazomethane, deprotected with trifluoroacetic acid-methylene chloride (1:1), and converted to hydrochloride γ-benzyl-L-glutamic acid α-methyl ester (γ-OBzl-L-Glu-α-OMe HCl) by treatment with dry hydrogen chloride in ethyl ether, MP: 129°-135°C (dec.), [α]D 25= + 13.3° (CHCl3).
Reaction of γ-OBzl-N-Boc-L-Glu with isobutyl chloroformate and Nmethylmorpholine followed by addition of γ-OBzl-L-Glu-α-OMe HCl afforded dipeptide γ-OBzl-N-Boc-L-Glu-γ-OBzl-L-Glu-α-OMe, yield 93%, MP: 58.5°- 60°C, [α]D 25= + 7.10° (CHCl3). After cleaving (trifluoroacetic acid-methylene chloride) the Boc group of above dipeptide, N-Tfa-L-Glu-OMe (Tfatrifluoroacetyl) was condensed with the product to afford tripeptide N-(γ-NTfa- L-Glu-α-OMe)-v-OBzl-N-Boc-L-Glu-γ-OBzl-L-Glu-α-OMe as colorless crystals, MP: 120°-122°C, [α]D 25= + 7.7° (CHCl3). Following hydrogenolysis of benzyl esters using palladium-on-carbon (10%), diacid N-(γ-N-Tfa-L-Glu-α- OMe)-L-Glu-L-GluOMe was obtained in yield 94% as colorless crystals, MP: 181°-184°C, [α]D 25= - 37.6° (CH3OH).
Selectively protected above diacid was converted to (γ-N-Tfa-L-Glu-α-OMe)-LGlu- L-Glu-tetra-OMe next way. To a solution of N-(γ-N-Tfa-L-Glu-α-OMe)-L-Glu- L-Glu-α-OMe (0.18 g) in dry acetone (20 ml cooled to 0°C was added with magnetic stirring ethereal diazomethane (0.8 mole) until yellow color persisted. After evaporation of solvent, the colorless solid was recrystallized from methylene chloride-hexanes to afford 0.17 g (92%) of colorless crystals of (γ-N-Tfa-L-Glu-α-OMe)-L-Glu-L-Glu-tetra-OMe, MP: 150°-152°C, [α]D 25= - 8.5° (CHCl3). A 1.00 g (1.9 mmoles) of (γ-N-Tfa-L-Glu-α-OMe)-L-Glu-L-Glu-tetra-OMe was dissolved under argon in dry dimethoxyethane (30 ml) with warming and magnetic stirring, and triethylamine (0.55 ml, 3.97 mmoles) was added. The reaction mixture was cooled to -30°C (dry ice isopropyl alcohol), and oxalyl chloride (0.35 ml, 3.97 mmoles) was added followed by dimethylformamide (2drops). The reaction mixture was warmed to 0°C by addition of hot isopropyl alcohol to the dry ice bath, stirred for 40 min, and then cooled to -78°C.
The cold acid chloride solution was slowly (30 min) added through the sintered-glass filter into ethereal solution of diazomethane (0.5 moles, 30 ml) cooled to -23°C (dry ice-carbon tetrachloride). After the mixture was stirred 30 min at -23°C and 30 min at 0°C, solvent was evaporated by steam argon, and the residue was chromatographed in 19:1 ethyl acetate-methanol on column of silica gel (70 g). The fraction (24:1 ethyl acetate-methanol) with TLC Rf 0.16 were collected and solvent evaporated to 0.30 g (38 %) of light yellow crystals of N-Tfa(-)-azotomycin-di-OMe, which upon recrystallization from chloroform-ethyl ether, melted at 134°-136°C, [α]D 25= - 22.9°.
To a mixture of N-Tfa(-)-azotomycin-di-OMe (106 mg, 0.18 mmoles) and methanol (0.12 ml) was added 1.0 N sodium hydroxide (0.83 ml, 8.3 mmoles) at room temperature and stirred for 30 min. Then the solution was acidified to pH 6.9 with 0.1 N hydrochloric acid and extracted with chloroform (2x25 ml). The aqueous phase was dissolved in methanol and passed through a column of Sephadex LH-20 (250 g), and fractions containing the magor product (TLC Rf 0.31, methanol, tailing) were collected. Solvent was evaporated and the residue was freeze-dried to yield 54.3 mg (65%) of (-)-azotomycin as a light yellow solid, which slowly decomposed at ambient temperature, [α]D 25= - 4.3°, IR, UV, 1H NMR, 13C NMR spectrum confirmed the structures of all described compounds.

Therapeutic Function

Antineoplastic

Safety Profile

Poison by ingestion,intraperitoneal, subcutaneous, and intravenous routes.Human gastrointestinal tract effects by intravenous route.When heated to decomposition it emits toxic fumes ofNOx.

Diazomycin B Preparation Products And Raw materials

Raw materials

Preparation Products

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Diazomycin B Suppliers

TargetMol Chemicals Inc.
Tel
+1-781-999-5354 +1-00000000000
Email
marketing@targetmol.com
Country
United States
ProdList
32165
Advantage
58
Dideu Industries Group Limited
Tel
+86-29-89586680 +86-15129568250
Email
1026@dideu.com
Country
China
ProdList
22883
Advantage
58
TargetMol Chemicals Inc.
Tel
15002134094
Email
marketing@targetmol.cn
Country
China
ProdList
19710
Advantage
58
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View Lastest Price from Diazomycin B manufacturers

Dideu Industries Group Limited
Product
Diazomycin B 7644-67-9
Price
US $1.10/g
Min. Order
1g
Purity
99.9%
Supply Ability
100 Tons Min
Release date
2021-07-01

7644-67-9, Diazomycin BRelated Search:


  • Azotomycin
  • Azotomycin sodium salt
  • Diazomycin B
  • DuazomycinB
  • 6-Diazo-N-(6-diazo-N-L-γ-glutamyl-5-oxo-L-norleucyl)-5-oxo-L-norleucine
  • Antibiotic-1719
  • NSC-56654
  • Antibiotic produced by streptomyces ambofaciens
  • Diazomycin B USP/EP/BP
  • 7644-67-9