ChemicalBook > CAS DataBase List > HYDROETHIDINE
HYDROETHIDINE
- Product Name
- HYDROETHIDINE
- CAS No.
- 38483-26-0
- Chemical Name
- HYDROETHIDINE
- Synonyms
- DHE;HYDROETHIDINE;reducedethidium;DIHYDROETHIDIUM;HYDROETHIDINE(TM);Dihydroethidium (DHE);ROS fluorescent probe DHE;Hydroethidine [Dihydroethidium] *CAS 38483-26-0*;5,6-dihydro-3,8-diamino-5-ethyl-6-phenylphenanthridine;3,8-DIAMINO-5,6-DIHYDRO-5-ETHYL-6-PHENYLPHENANTHRIDINE
- CBNumber
- CB2220169
- Molecular Formula
- C21H21N3
- Formula Weight
- 315.41
- MOL File
- 38483-26-0.mol
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HYDROETHIDINE Property
- Boiling point:
- 444.96°C (rough estimate)
- Density
- 1.1227 (rough estimate)
- refractive index
- 1.6380 (estimate)
- storage temp.
- −20°C
- solubility
- acetonitrile: soluble
- CAS DataBase Reference
- 38483-26-0(CAS DataBase Reference)
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Safety
- Hazard Codes
- Xn
- Risk Statements
- 36/37/38-22
- Safety Statements
- 37/39-26
- WGK Germany
- 3
- F
- 8-10
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N-Bromosuccinimide Price
Sigma-Aldrich
- Product number
- A0457
- Product name
- Anti-Activin Receptor IIB antibody produced in goat
- Purity
- affinity isolated antibody
- Packaging
- 0.1mg
- Price
- $568
- Updated
- 2024/03/01
Sigma-Aldrich
- Product number
- 309800
- Product name
- Dihydroethidium - CAS 38483-26-0 - Calbiochem
- Purity
- A cell-permeable, chemically-reduced ethidium derivative.
- Packaging
- 25mg
- Price
- $254
- Updated
- 2024/03/01
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HYDROETHIDINE Chemical Properties,Usage,Production
Uses
Dihydroethidium, also known as hydroethidine, is a dye that can permeate viable cells and accumulate in the nucleus after it is dehydrogenated to ethidium bromide and is used to measure oxygen reduction in phagocytes during respiratory burst. In the presence of an oxidant it will fluoresce and has been used as a dye in flow cytometry. Studies have used Dihydroethidium to study alveolar macrophages, blood leukocytes, and epithelial cells, among others, and has be used to measure intracellular H2O2 and O2-, and reactive oxygen. Dyes and metabolites.
General Description
A cell-permeable, chemically reduced ethidium derivative that can be used as a fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, however, following oxidation to ethidium and subsequent intercalation to DNA, a bright red fluorescence is achieved. Often used to determine the level of superoxide anion in cells by flow cytometry.
Biochem/physiol Actions
Cell permeable: yes
HYDROETHIDINE Preparation Products And Raw materials
Raw materials
Preparation Products
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