DM4 Property

Melting point:

185-187 °C (decomp)

Boiling point:

943.2±65.0 °C(Predicted)

Density 

1.29±0.1 g/cm3(Predicted)

storage temp. 

Store at -20°C

solubility 

DMSO : 100 mg/mL (128.14 mM)

form 

Solid

pka

9.82±0.70(Predicted)

color 

White to light yellow

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H300Fatal if swallowed

H310Fatal in contact with skin

H314Causes severe skin burns and eye damage

H340May cause genetic defects

H360May damage fertility or the unborn child

H370Causes damage to organs

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P262Do not get in eyes, on skin, or on clothing.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P301+P310IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.

P301+P330+P331IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.

P302+P350IF ON SKIN: Gently wash with plenty of soap and water.

P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P307+P311IF exposed: call a POISON CENTER or doctor/physician.

P310Immediately call a POISON CENTER or doctor/physician.

P321Specific treatment (see … on this label).

P322Specific measures (see …on this label).

P330Rinse mouth.

P361Remove/Take off immediately all contaminated clothing.

P363Wash contaminated clothing before reuse.

P405Store locked up.

P501Dispose of contents/container to..…

DM4 Chemical Properties,Usage,Production

Structure

Structurally, maytansinoid derivatives DM1, DM3, and DM4 differ based on the number of methyl groups present on the carbon adjacent to sulfur—zero, one, or two, respectively. The introduction of methyl groups contributes to the heightened potency of the free drug, likely due to increased hydrophobicity and improved membrane permeability. DM4 is a thiol-containing compound that undergoes methylation within cells through the activity of methyltransferases, forming S-Me-DM4. This metabolite demonstrates notable cytotoxicity. Importantly, both DM4 and S-Me-DM4 can pass through cell membranes, leading to an enhanced phenomenon known as the "bystander effect," where the drug can diffuse out of a targeted cell into adjacent cells[1].

Description

Ravtansine (DM4) is a maytansinoid, a chemical derivative of maytansine being investigated as the cytotoxic payload of a number of antibody-drug conjugates (ADCs).M4 is is an antitubulin agent that inhibit cell division. DM4 can be used in the preparation of antibody drug conjugate.

Uses

N2''-Deacetyl-N2''-(4-mercapto-4-methyl-1-oxopentyl)-maytansine is an intermediate used to prepare semisynthetic maytansine analogs which can be conjugated with antibodies for the targeted treatment of cancer.

Biological Activity

The anticancer properties of maytansinoids have been attributed to their ability to disrupt microtubule function. The maytansinoid emtansine (DM1), for example, binds at the ends of microtubules and thereby suppress their dynamic instability. Other maytansinoids include: Ansamitocin; Mertansine/emtansine (DM1); Ravtansine/soravtansine (DM4).

Synthesis

A solution of N-methyl-N-(4-methyldithio-4-methyl-1-oxopropyl)-L-alanine (36.6 mg, 0.313 mmol) and 1,3-dicyclohexylcarbodiimide (13.51 mg, 0.065 mmol) was prepared in CH2Cl2 (0.5 mL) in a round bottom flask equipped with a stir bar and maintained under an argon atmosphere. The solution stirred vigorously at room temperature for 30 minutes as the symmetrical anhydride formed. The reaction mixture was filtered through glass wool and added to a reaction flask containing maytansinol (12.3 mg, 0.022 mmol) and zinc bis[bis(trimethylsilyl)amide] (42.5 mg, 0.109 mmol) prepared in CH2Cl2 (1 mL). The reaction proceeded at room temperature under an argon atmosphere with stirring. After 3 hours, an additional 5 equivalents of zinc bis[bis(trimethylsilyl)amide] (42.5 mg, 0.109 mmol) were added to the reaction vessel and the reaction proceeded overnight. Analytical thin layer chromatography (Analtech Uniplate, 2.5?á10 cm, 250 micron), eluting in a mixture of methylene chloride and methanol (95:5, v/v) indicated the formation of the desired N-methyl-L-alanyl ester of maytansinol at C3. The degree of conversion of the reaction was determined by analytical HPLC analysis using a Vydac protein & peptide C18 column (4.6?á250 mm) at a flow rate of 1.50 mL/min, eluting with a gradient of water and acetonitrile, as follows: Time (min) % A (water) % B (acetonitrile) 0 95 5 2.5 95 5 32 5 95 35 5 95 Under these conditions the thiol containing product eluted at 20.75 min with no detected D-isomer. MS: m/z found 780.8 (M+Na+) calculated 781.4.

in vitro

DM4, a structural analogue of maytansine, is a new thiol-containing and potent maytansinoid. DM4 is a cytotoxic maytansinoid drug. It is synthesized in order to link maytansinoids to antibodies via disulfide bonds. Maytansinoids inhibit tubulin polymerization and microtubule assembly and enhance microtubule destabilization, so there is potent suppression of microtubule dynamics resulting in a mitotic block and subsequent apoptotic cell death.

References

[1] Giulio Lovato . “HPLC-DAD validated method for DM4 and its metabolite S-Me-DM4 quantification in biological matrix for clinical and pharmaceutical applications.” Journal of pharmaceutical and biomedical analysis 235 (2023): Article 115642.