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sodium 8-amino-5-chloro-1-oxo-7-phenyl-2H-pyrido[3,4-d]pyridazin-4-olate

Product Name
sodium 8-amino-5-chloro-1-oxo-7-phenyl-2H-pyrido[3,4-d]pyridazin-4-olate
CAS No.
143556-24-5
Chemical Name
sodium 8-amino-5-chloro-1-oxo-7-phenyl-2H-pyrido[3,4-d]pyridazin-4-olate
Synonyms
L 012 sodium salt;L 012 sodium salt, 10 mM in DMSO;1H-Benz[e]indole-6,8-disulfonic acid, 1,1,2-trimethyl-;sodium 8-amino-5-chloro-1-oxo-7-phenyl-2H-pyrido[3,4-d]pyridazin-4-olate;8-Amino-5-chloro-2,3-dihydro-7-phenyl-pyrido[3,4-d]pyridazine sodium salt;8-Amino-5-chloro-7-phenyl-2,3-dihydropyrido[3,4-d]pyridazine-1,4-dione sodium salt
CBNumber
CB23414213
Molecular Formula
C13H8ClN4NaO2
Formula Weight
310.67099
MOL File
143556-24-5.mol
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sodium 8-amino-5-chloro-1-oxo-7-phenyl-2H-pyrido[3,4-d]pyridazin-4-olate Property

storage temp. 
-20°C
solubility 
Soluble to 20 mM in water and to 100 mM in DMSO
form 
Solid
color 
White to yellow
Water Solubility 
H2O: 2mg/mL, clear
InChI
InChI=1S/C13H9ClN4O2.Na/c14-11-8-7(12(19)17-18-13(8)20)9(15)10(16-11)6-4-2-1-3-5-6;/h1-5H,15H2,(H,17,19)(H,18,20);/q;+1/p-1
InChIKey
IGEUYSJHQQCEFP-UHFFFAOYSA-M
SMILES
[Na+].C1([O-])=NNC(=O)C2=C(N)C(C3=CC=CC=C3)=NC(Cl)=C12
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P321Specific treatment (see … on this label).

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P362Take off contaminated clothing and wash before reuse.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
SML2236
Product name
L-012 sodium salt
Purity
≥98% (HPLC)
Packaging
5mg
Price
$134
Updated
2025/07/31
Sigma-Aldrich
Product number
SML2236
Product name
L-012 sodium salt
Purity
≥98% (HPLC)
Packaging
25mg
Price
$511.1
Updated
2025/07/31
Tocris
Product number
5085
Product name
L-012 sodium salt
Purity
≥98%(HPLC)
Packaging
10
Price
$278
Updated
2021/12/16
TRC
Product number
L473478
Product name
L-012 sodium salt
Packaging
10mg
Price
$255
Updated
2021/12/16
Usbiological
Product number
255621
Product name
L 012 sodium salt
Packaging
10mg
Price
$579
Updated
2021/12/16
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sodium 8-amino-5-chloro-1-oxo-7-phenyl-2H-pyrido[3,4-d]pyridazin-4-olate Chemical Properties,Usage,Production

Description

L 012 sodium salt is a luminal-based chemiluminescent dye and probe. It is used to detect superoxide in biological systems, specifically in whole inflammatory cells, vascular tissue and blood. It could detect NAPDH oxidase-derived reactive oxygen and nitrogen species (ROS and RNS). It is active in vitro and in vivo.

Uses

L 012 Sodium Salt is a chemiluminescent dye used to detect superoxide in biological systems, specifically in whole blood, inflammatory cells, and vascular tissue.

Biological Activity

L-012 is a luminol analog and a widely used reactive oxygen and nitrogen species (RONS; ROS & RNS) chemiluminescence (CL) probe both in cultures and in animals in vivo. L-012 displays significantly higher CL yield and sensitivity than luminol, lucigenin and MCLA. Instead of reacting with superoxide anion (O2-) directly, L-012 (LumH2) is converted via a one-electron oxidation (catalyzed by peroxidase in the presence of H2O2) to the LumH· radical form, which then reacts with oxygen (O2) to yield O2- and L-012 quinone (q-Lum). The O2- in turn reacts with the LumH· radical, leading eventually to an endoperoxide th at decomposes to emit luminescence. To a less extend, H2O2 can also react with q-Lum to yield luminescence. Although not specific to NADPH oxidase-derived RONS, L-012 is also commonly used for screening NADPH oxidase inhibitors.

in vivo

L-012 sodium salt is well distributed in the mouse body and mediates a strong ROS/RNS-dependent luminescent signal in vivo and is useful for monitoring the development and regulation of inflammation in living organisms[2].

storage

Store at -20°C

sodium 8-amino-5-chloro-1-oxo-7-phenyl-2H-pyrido[3,4-d]pyridazin-4-olate Preparation Products And Raw materials

Raw materials

Preparation Products

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sodium 8-amino-5-chloro-1-oxo-7-phenyl-2H-pyrido[3,4-d]pyridazin-4-olate Suppliers

Chongqing Chemieliva Pharmaceutical Co., Ltd.
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18696571988
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+86 (23) 6777-0220
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amy.lei@chemieliva.com
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China
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Nanjing Datang Pharmaceutical Technology Co., Ltd.
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025-84341491 13951930983
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025-84341491
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China
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Shanghai Macklin Biochemical Co.,Ltd.
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15221275939
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021-50706099
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021-65675885 18964387627
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Zhengzhou Alfa Chemical Co.,Ltd
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+8618530059196
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TargetMol Chemicals Inc.
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+1-781-999-5354; +17819995354
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marketing@targetmol.com
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United States
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Henan Weituxi Chemical Technology Co., Ltd.
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0371-63284658 18135795563
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2885349392@qq.com
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View Lastest Price from sodium 8-amino-5-chloro-1-oxo-7-phenyl-2H-pyrido[3,4-d]pyridazin-4-olate manufacturers

ZHENGZHOU JIUYI TIME NEW MATERIALS CO,.LTD
Product
L 012 sodium salt 143556-24-5
Price
US $3.90-5.90/KG
Min. Order
1KG
Purity
99%
Supply Ability
g-kg-tons, free sample is available
Release date
2025-06-12

143556-24-5, sodium 8-amino-5-chloro-1-oxo-7-phenyl-2H-pyrido[3,4-d]pyridazin-4-olateRelated Search:


  • sodium 8-amino-5-chloro-1-oxo-7-phenyl-2H-pyrido[3,4-d]pyridazin-4-olate
  • 1H-Benz[e]indole-6,8-disulfonic acid, 1,1,2-trimethyl-
  • 8-Amino-5-chloro-2,3-dihydro-7-phenyl-pyrido[3,4-d]pyridazine sodium salt
  • L 012 sodium salt
  • 8-Amino-5-chloro-7-phenyl-2,3-dihydropyrido[3,4-d]pyridazine-1,4-dione sodium salt
  • L 012 sodium salt, 10 mM in DMSO
  • 143556-24-5
  • C13H8CIN4NaO2