ChemicalBook > CAS DataBase List > FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5

FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5

Product Name: FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5
CAS No.: 273221-67-3
Chemical Name: FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5
Synonyms: Fluo 4-AM;HY-101896;Fluo4amester;Fluo-4-(acetoxymethyl) ester;Fluo-4 AM, Fluorescent labeling reagent;FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5;Fluo-4 AM, Calcium Ion Fluorescent Probe;Fluo-4 AM Calcium Fluorescent Probes (Green);Fluo-4 AM *Ultrapure Grade* *CAS 273221-67-3*;4-(6-acetoxymethoxy-2,7-difluoro-3-oxo-9-xanthenyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-n,n,n',n'-tetraacetic acid tetrakis(acetoxymethyl) ester
CBNumber: CB2497293
Molecular Formula: C51H50F2N2O23
Formula Weight: 1096.94
MOL File: 273221-67-3.mol

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FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 Property

Melting point:: >200℃
Boiling point:: 1063.2±65.0 °C(Predicted)
Density: 1.47
storage temp.: −20°C
solubility: DMSO: soluble
pka: 1.92±0.50(Predicted)
form: Solid
color: Brown to reddish brown
Appearance: orange-red powder
λmax: 456nm
Biological Applications: Calcium indicator
InChIKey: QOMNQGZXFYNBNG-UHFFFAOYSA-N
SMILES: C(OCOC(C)=O)(=O)CN(C1=CC=C(C2C3=C(C=C(OCOC(C)=O)C(F)=C3)OC3C=2C=C(F)C(=O)C=3)C=C1OCCOC1=CC(C)=CC=C1N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O

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Safety

Risk Statements: 36/37/38
Safety Statements: 26-36
WGK Germany: 3
F: 10-21
HS Code: 32049010

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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Tocris

Product number: 6255
Product name: Fluo-4AM
Purity: ≥95%(HPLC)
Packaging: 1
Price: $381
Updated: 2021/12/16

TRC

Product number: F647558
Product name: Fluo-4AM
Packaging: 5mg
Price: $1765
Updated: 2021/12/16

ChemScene

Product number: CS-7990
Product name: Fluo-4AM
Purity: ≥99.0%
Packaging: 100ug
Price: $150
Updated: 2021/12/16

American Custom Chemicals Corporation

Product number: CHM0152903
Product name: FLUO-4-(ACETOXYMETHYL) ESTER
Purity: 95.00%
Packaging: 5MG
Price: $497.92
Updated: 2021/12/16

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FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 Chemical Properties,Usage,Production

Description

Fluo-4 AM is a cell-permeable Ca²⁺-indicator that is metabolized by intracellular esterase, leading to a bright green fluorescent signal upon Ca²⁺-binding (excitation/emission λ at 494/506 nm). Fluo-4 AM is used for visualization and measurement of intracellular Ca²⁺. It is well suited for fluorometric and imaging applications such as microscopy, flow cytometry, spectrofluorometry, and fluorometric high-throughput microplate screening assays [1].

Fluo-4 AM is similar in structure and spectral properties to the widely used Ca²⁺-indicator, Fluo-3, but it has certain advantages over Fluo-3, such as brighter fluorescence emission, high rate of cell permeation, and a K_d for Ca²⁺ in buffer of 345 nM. Because of its higher fluorescence emission intensity, Fluo-4 AM can be used at lower intracellular concentrations, making its use less toxic for live cells.

As Fluo-4 AM does not covalently bind to cellular components, it may be actively effluxed from the cell by organic anion transporters. In vivo cell imaging with Fluo-4 AM is usually performed within one or two hours after loading, but the dye can be re-loaded to cells if it is needed. Fluo-4 AM can also be fixed in situ by EDC/EDAC for downstream immunofluorescence studies.

Fluo-4 AM has low solubility in the water. It is recommended to prepare 1 mM stock solution in labeling grade DMSO prior to cell loading. Use the final concentration of 1-5 μM and incubation at 37 °C for 15-60 min as a start point of your protocol.

Uses

Fluorescent Ca2+ indicator dye; cell-permeable AM ester, insensitive to magnesium.

Uses

Fluo-4 AM is a cell-permeable, fluorescent Ca2+ indicator. Displays high fluorescence emission. Excitation/emission λ 494/516 nm.

storage

Store at -20°C

Abs/Em Maxima

494/518 nm

Excitation / Emission Maximum

494 nm/506 nm

FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 Preparation Products And Raw materials

Raw materials

Preparation Products

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273221-67-3, FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5Related Search:

FLUOZIN 2 FLUOZIN 3 FLUOZIN 1 Fluo 4 Fluo 3-AM FLUO 3

FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5

Fluo 4-AM

4-(6-acetoxymethoxy-2,7-difluoro-3-oxo-9-xanthenyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-n,n,n',n'-tetraacetic acid tetrakis(acetoxymethyl) ester

N-[4-[6-[(Acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine (acetyloxy)methyl ester

Fluo4amester

Fluo-4 AM *Ultrapure Grade* *CAS 273221-67-3*

Fluo-4-(acetoxymethyl) ester

Glycine, N-[4-[6-[(acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-, (acetyloxy)methyl ester

1-[2-Amino-5-(2,7-difluoro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethyl ester

Fluo-4 AM, Fluorescent labeling reagent

1-[2-Amino-5-(2,7-difluoro-6-acetoxymethoxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, tetra(acetoxymethyl)ester

HY-101896

Fluo-4 AM Calcium Fluorescent Probes (Green)

Fluo-4 AM, Calcium Ion Fluorescent Probe

273221-67-3

C51H50F2N2O23

Xanthene