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FLAG peptide

Product Name
FLAG peptide
CAS No.
98849-88-8
Chemical Name
FLAG peptide
Synonyms
FLAG®FLAG tag Peptide;FLAG PEPTIDE, >98%;FLAG Peptide TFA salt;FLAG Tag Peptide,FLAG epitope;Inhibitor,FLAG peptide,inhibit;FLAG PEPTIDE|DYKDDDDK|ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS;FLAG peptide,DYKDDDDK,Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, >98%;(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-amino-3-carboxypropanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]hexanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]hexanoic acid
CBNumber
CB31395616
Molecular Formula
C41H60N10O20
Formula Weight
1012.97
MOL File
98849-88-8.mol
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FLAG peptide Property

Density 
1.470±0.06 g/cm3 (20 ºC 760 Torr)
storage temp. 
Keep in dark place,Sealed in dry,Store in freezer, under -20°C
solubility 
≥50.65 mg/mL in DMSO; ≥210.6 mg/mL in H2O; ≥34.03 mg/mL in EtOH
form 
Lyophilized powder
Sequence
H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH
InChI
InChI=1/C41H60N10O20/c42-11-3-1-5-22(45-36(65)24(13-19-7-9-20(52)10-8-19)47-34(63)21(44)14-29(53)54)35(64)48-26(16-31(57)58)38(67)50-28(18-33(61)62)40(69)51-27(17-32(59)60)39(68)49-25(15-30(55)56)37(66)46-23(41(70)71)6-2-4-12-43/h7-10,21-28,52H,1-6,11-18,42-44H2,(H,45,65)(H,46,66)(H,47,63)(H,48,64)(H,49,68)(H,50,67)(H,51,69)(H,53,54)(H,55,56)(H,57,58)(H,59,60)(H,61,62)(H,70,71)/t21-,22-,23-,24-,25-,26-,27-,28-/s3
InChIKey
XZWYTXMRWQJBGX-OVIGALBNNA-N
SMILES
[C@@H](NC(=O)[C@@H](N)CC(=O)O)(C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@H](C(=O)O)CCCCN)CC1=CC=C(O)C=C1 |&1:0,4,13,22,30,38,46,54,r|
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Alfa Aesar
Product number
J66909
Product name
Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
Packaging
1mg
Price
$87.3
Updated
2023/06/20
Alfa Aesar
Product number
J66909
Product name
Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
Packaging
0.5mg
Price
$32
Updated
2021/12/16
ChemScene
Product number
CS-6485
Product name
FLAGpeptide
Purity
99.23%
Packaging
1mg
Price
$50
Updated
2021/12/16
ChemScene
Product number
CS-6485
Product name
FLAGpeptide
Purity
99.23%
Packaging
5mg
Price
$70
Updated
2021/12/16
ApexBio Technology
Product number
A6002
Product name
DYKDDDDKtagPeptide
Packaging
4mg
Price
$70
Updated
2021/12/16
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FLAG peptide Chemical Properties,Usage,Production

Definition

ChEBI: FLAG peptide is an eight amino acid peptide consisting of L-aspartic acid, L-tyrosine, L-lysine, four L-aspartic acid residues, and L-lysine joined in sequence by peptide linkages. It is widely used as a fusion tag for the purification and detection of a wide variety of recombinant proteins.

Biological Activity

a fusion tag called dykddddk and consisting of eight amino acids asp-tyr-lys-asp-asp-asp-asp-lys including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. it allows elution under non-denaturing conditions. several antibodies against this peptide have been developed. one antibody, denoted as m1, binds the peptide in the presence of bivalent metal cations, preferably ca+. elution is effected by chelating agents. another strategy is competitive elution with excess of free dykddddk peptide. antibodies m2 and m5 are applied in this procedure1.the added marker segment is that it should not interfere with the native folding of proteins to which it is attached. secondly, the marker peptide sequence should be water-soluble and should retain a high degree of exposure on the surface of the protein, so that it can readily interact with its ligand. it should also be suitable for a mild and inexpensive affinity purification procedure. finally, an easy removal of the marker peptide leading to a native product is also advantageous. due to this small size, the marker peptide can be encoded by a single synthetic oligonucleotide. it is known that aromatic amino acids are the major factors in antigen–antibody interactions.lys at position 3 in the marker sequence leads to a hexapeptide sequence lysaspaspaspasplys, which ensures a maximum value on the hydrophilicity scale according to hopp and woods2 . such hydrophilic sequences have been shown to express strong antigenicity and are thus likely to adopt a highly exposed conformation in the three-dimensional folding of proteins3. another virtue of dykddddk is that the longest trypsinogen prosequences are of this length. this enables the removal of the tag and the production of an authentic n-terminus of the fusion protein partner by enterokinase treatment. the dykddddk peptide can be fused to either the n- or c-terminus of a given fusion protein. nevertheless, the n-terminal fusion has several advantages. inhibition elisa experiments showed that the anti-dykddddk antibody m1 binds three to four orders of magnitude better under conditions where the a-amino group of the first amino acid is freely accessible4.the dykddddk marker peptide fusion system comprises a unique and widely useful technique for protein identification and purification. elution of the fusion protein can be accomplished either by antibody-mediated affinity chromatography in a calcium-dependent manner, by lowering the ph, or by competitive elution with synthetic peptides1. although highly selective, the binding capacities are low, making scale-up a costly undertaking. in addition to cost and low capacity, large-scale immunoaffinity chromatography, applied to the production of therapeutic proteins has several disadvantages: ligand leakage, instability, and need for validation of antibody production. the stability of the affinity chromatography column depends on the nature and source of the crude extracts. furthermore, the dykddddk tag, designed to be immunogenic, must be removed from therapeutic proteins. in most cases, this can be accomplished with enterokinase. however, contaminating proteases may also produce undesired cleavages. despite these drawbacks, the dykddddk fusion is useful in research and development: dykddddk proteins can be readily purified and assayed by elisa or any other immunochemical detection method, thus expediting the raising of antisera against a desired protein and characterization studies.

References

1. a. einhauer, a. jungbauer. the flag peptide, a versatile fusion tag for the purification of recombinant proteins. j. biochem. biophys. methods 49 2001 455–465. 2. hopp tp, woods kr. prediction of protein antigenic determinants from amino acid sequences. proc natl acad sci u s a 1981;78:3824–8.3. hopp tp. protein surface analysis: methods for identifying antigenic determinants and other interaction sites. j immunol methods 1986;88:1–18.4. power be, ivancic n, harley vr, webster rg, kortt aa, irving ra, et al. high-level temperature-induced synthesis of an a

FLAG peptide Preparation Products And Raw materials

Raw materials

Preparation Products

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FLAG peptide Suppliers

Taiyuan Bailihengkang Biotechnology Co., Ltd
Tel
19135651983 19135651983
Email
18611134419@163.com
Country
China
ProdList
4976
Advantage
58
GL Biochem (Shanghai) Ltd
Tel
21-61263452 13641803416
Fax
86-21-61263399
Email
ymbetter@glbiochem.com
Country
China
ProdList
9981
Advantage
64
Shanghai Hanhong Scientific Co.,Ltd.
Tel
Country
China
ProdList
42982
Advantage
64
Chemsky (shanghai) International Co.,Ltd
Tel
021-50135380
Email
shchemsky@sina.com
Country
China
ProdList
15421
Advantage
60
Shandong Xiya Chemical Co., Ltd
Tel
13355009207 13355009207
Fax
0539-6365991
Email
3007715519@qq.com
Country
China
ProdList
18739
Advantage
57
Dalian Meilun Biotech Co., Ltd.
Tel
0411-62910999 13889544652
Email
sales@meilune.com
Country
China
ProdList
4654
Advantage
58
AdooQ BioScience, LLC
Tel
+1 (866) 930-6790
Fax
+1 (866) 333-9607
Email
info@adooq.com
Country
United States
ProdList
2784
Advantage
58
ApexBio Technology LLC
Tel
+1-832-696-8203
Fax
+1-832-641-3177
Email
info@apexbt.com
Country
United States
ProdList
477
Advantage
60
AdooQ Bioscience CHINA
Tel
025-58849295 18951903616;
Fax
025-68650336
Email
info@adooq.cn
Country
China
ProdList
2989
Advantage
60
Creative Peptides
Tel
Email
info@creative-peptides.com
Country
United States
ProdList
6084
Advantage
56
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View Lastest Price from FLAG peptide manufacturers

Dorne Chemical Technology co. LTD
Product
FLAG peptide 98849-88-8
Price
US $1.00/g
Min. Order
1g
Purity
99%
Supply Ability
100kg
Release date
2024-03-26
Shanghai Affida new material science and technology center
Product
FLAG peptide 98849-88-8
Price
US $0.00-0.00/Box
Min. Order
1Box
Purity
99
Supply Ability
50000KG/month
Release date
2024-04-26
Shandong Hanjiang Chemical Co., Ltd
Product
flag 98849-88-8
Price
US $0.00/kg
Min. Order
1kg
Purity
0.99
Supply Ability
10T
Release date
2024-01-11

98849-88-8, FLAG peptideRelated Search:


  • FLAG tag Peptide
  • FLAG PEPTIDE|DYKDDDDK|ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS
  • FLAG Tag Peptide,FLAG epitope
  • FLAG&reg
  • FLAG PEPTIDE, >98%
  • FLAG peptide,DYKDDDDK,Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, >98%
  • (2S)-6-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-amino-3-carboxypropanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]hexanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]hexanoic acid
  • Inhibitor,FLAG peptide,inhibit
  • FLAG Peptide TFA salt
  • 98849-88-8
  • peptides
  • EpitopeTags