DEMECOLCINE
- Product Name
- DEMECOLCINE
- CAS No.
- 477-30-5
- Chemical Name
- DEMECOLCINE
- Synonyms
- COLCEMID;COLCHAMINE;(S)-1,2,3,10-Tetramethoxy-7-(methylamino)-6,7-dihydrobenzo[a]heptalen-9(5H)-one;omain;Osamin;omaine;C-12669;nsc3096;Colcemide;ColchaMin
- CBNumber
- CB3460085
- Molecular Formula
- C21H25NO5
- Formula Weight
- 371.43
- MOL File
- 477-30-5.mol
DEMECOLCINE Property
- Melting point:
- 73-75°C
- alpha
- D20 -129.0° (c = 1 in chloroform)
- Boiling point:
- 501.11°C (rough estimate)
- Density
- 1.2350 (rough estimate)
- refractive index
- 1.5614 (estimate)
- storage temp.
- 2-8°C
- solubility
- DMSO: 10 mg/mL
- form
- powder
- pka
- 8.48±0.40(Predicted)
- Sensitive
- Air & Light Sensitive
- BRN
- 2822892
- Stability:
- Hygroscopic
Safety
- Hazard Codes
- T,T+
- Risk Statements
- 25-26/27/28
- Safety Statements
- 22-24/25-45-36/37/39
- RIDADR
- UN 2811 6.1/PG 2
- WGK Germany
- 3
- RTECS
- GH0800000
- F
- 8-10-23
- HazardClass
- 6.1(a)
- PackingGroup
- II
- Toxicity
- LD50 oral in mouse: 25530ug/kg
N-Bromosuccinimide Price
- Product number
- D1925
- Product name
- Demecolcine solution
- Purity
- 10 μg/mL in HBSS, ACF Qualified, BioXtra
- Packaging
- 20ml
- Price
- $62.7
- Updated
- 2024/03/01
- Product number
- 234109-M
- Product name
- Colcemid - CAS 477-30-5 - Calbiochem
- Purity
- Cell synchronization agent.
- Packaging
- 5mg
- Price
- $101
- Updated
- 2024/03/01
- Product number
- 10295892001
- Product name
- Colcemid
- Purity
- N-methyl-N-deacetyl-colchicine
- Packaging
- 20ml
- Price
- $60.6
- Updated
- 2024/03/01
- Product number
- J63900
- Product name
- Colcemid
- Purity
- 98+%
- Packaging
- 10mg
- Price
- $222
- Updated
- 2023/06/20
- Product number
- J63900
- Product name
- Colcemid
- Packaging
- 50mg
- Price
- $914
- Updated
- 2023/06/20
DEMECOLCINE Chemical Properties,Usage,Production
Description
Colcemid is a colchicine derivative that inhibits tubulin polymerization as potently as colchicine (IC50 = 2.1 and 2.4 μM, respectively) but is less toxic. At very low (nanomolar) concentrations, colcemid suppresses microtubule dynamicity and inhibits cell migration, while at micromolar levels it blocks microtubule assembly, arresting cells in metaphase. Mitotic block by colcemid is used to synchronize cells and for karyotyping in cytogenetic studies. Prolonged exposure to colcemid can activate p53, leading to apoptosis.
Chemical Properties
Faintly Yellow Crystalline Powder
Uses
An antimitotic agent that disrupts microtubles by binding to tubulin and preventing its polymerization. Stimulates the intrinsic GTPase activity of tubulin. Induces apoptosis in several normal and tumor cell lines and activates the JNK/SAPK signaling pathway.
Uses
Inhibitor of spindle fiber formation
Uses
Cell synchronization agent; for chromosome visualization; to induce oocyte enucleation for somatic cell cloning.
Definition
ChEBI: A secondary amino compound that is (S)-colchicine in which the N-acetyl group is replaced by an N-methyl group. Isolable from the autumn crocus, Colchicum autumnale, it is less toxic than olchicine and is used as an antineoplastic.
General Description
Colcemid is also known as demecolcine. Its generic name is N-methyl-N-deacetyl-colchicine. Colcemid depolymerizes microtubules and blocks mitosis at metaphase.
Biochem/physiol Actions
Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.
Safety Profile
Poison by ingestion, intraperitoneal, parenteral, intravenous, and intramuscular routes. Human systemic effects by ingestion: (skin and appendages) hair effects. Human mutation data reported. An experimental teratogen. Experimental reproductive effects. When heated to decomposition it emits toxic fumes of NOx.
Purification Methods
Colcemide is purified by chromatography on silica and eluting with CHCl3/MeOH (9:1), and by recrystallisation from EtOAc/Et2O to form yellow prisms. UV in EtOH has max 243nm ( 30,200) and 350nm ( 16,3000). [Synthesis, IR, NMR, MS: Capraro & Brossi Helv Chim Acta 62 965 1979, Beilstein 8 IV 3319.]
DEMECOLCINE Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from DEMECOLCINE manufacturers
- Product
- DEMECOLCINE 477-30-5
- Price
- US $8.00-1.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- g-kg-tons, free sample is available
- Release date
- 2024-04-09
- Product
- Demecolcine 477-30-5
- Price
- US $130.00/T
- Min. Order
- 1Kg/Bag
- Purity
- 99.99%
- Supply Ability
- 20 tons/month
- Release date
- 2020-12-24
- Product
- DEMECOLCINE 477-30-5
- Price
- US $1.10/g
- Min. Order
- 1g
- Purity
- 99.9%
- Supply Ability
- 100 Tons Min
- Release date
- 2021-07-02