DABSYL CHLORIDE Property

Melting point:

185 °C (dec.) (lit.)

Boiling point:

483.6±30.0 °C(Predicted)

Density 

1.29±0.1 g/cm3(Predicted)

storage temp. 

room temp

solubility 

DMF: soluble

pka

3.27±0.10(Predicted)

form 

powder to crystaline

color 

Orange to Brown to Dark red

Sensitive 

Moisture Sensitive

BRN 

3064095

CAS DataBase Reference

56512-49-3(CAS DataBase Reference)

Safety

Hazard Codes 

C

Risk Statements 

34

Safety Statements 

26-36/37/39-45-27

RIDADR 

UN 3261 8/PG 2

WGK Germany 

3

RTECS 

DB8927800

F 

10-21

HazardClass 

8

PackingGroup 

II

HS Code 

29270000

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H314Causes severe skin burns and eye damage

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

DABSYL CHLORIDE Chemical Properties,Usage,Production

Chemical Properties

Dark red crystalline

Uses

For the qualitative and quantitative identification of amino acids and for the determination of N-terminal amino acids. Used in the determination of primary and secondary amines by HPLC.

General Description

Amino groups are common to many biological compounds and many amines used in pharmaceuticals. Most of these compounds show weak absorption in direct HPLC analysis with UV-VIS detection. However, strongly absorbing derivatives of amines can be easily detected by HPLC with high sensitivity even when present at low concentrations. Primary and secondary amino groups are usually derivatized as aromatic derivatives by nucleophilic substitution reactions using reagents such as arylsulfonyl chlorides.
4-(Dimethylamino)azobenzene-4′-sulfonyl chloride, a well-known UV-labeling agent, is generally used for the derivatization of N-terminal amino acids, imidazole derivatives, polyamines, etc. prior to their chromatographic determination due to the former′s capacity to covalently bind to the substrates. Its derivatives typically absorb UV radiation between the range of 436-460 nm. This method of derivatization is regarded as superior compared to other methods owing to the simplicity of the procedure, high stability and reproducibility, best resolution of the analytes, etc.