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CTC

Product Name
CTC
CAS No.
90217-02-0
Chemical Name
CTC
Synonyms
CTC;Ctc (biological Stain);Cyanotolyl Tetrazolium, Ctc;CYANODITOLYL TETRAZOLIUM CHLORIDE;CYANOTOLYL TETRAZOLIUM CHLORIDE (CTC);5-CYANO-2,3-DITOLYL TETRAZOLIUM CHLORIDE;5-Cyano-2,3-ditolyl-2H-tetrazolium chloride;5-CYANO-2,3-DI-(P-TOLYL)TETRAZOLIUM CHLORIDE;CTC 5-Cyano-2,3-ditolyl tetrazolium chloride;5-Cyano-2,3-di-p-tolyl-2H-tetrazol-3-ium Chloride
CBNumber
CB4169851
Molecular Formula
C16H14ClN5
Formula Weight
311.77
MOL File
90217-02-0.mol
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CTC Property

Melting point:
225 ℃
storage temp. 
2-8°C
solubility 
Soluble in water
form 
powder
color 
Off-white
λmax
450nm (of formazan)
BRN 
8954451
Biological Applications
NADPH-cytochrome P 450 reductase activity assays; detecting gamma-hydroxybutyric acid (GHB),metabolic activity,microorganisms; localizing glucose-6-phosphate dehydrogenase activity; monitoring respiratory activity; treating cancer
InChI
InChI=1S/C16H15N5.ClH/c1-13-3-7-15(8-4-13)19-12-18-20(11-17)21(19)16-9-5-14(2)6-10-16;/h3-10,12H,1-2H3;1H
InChIKey
BLCCXPMHHCWJHC-UHFFFAOYSA-N
SMILES
N1(C2=CC=C(C)C=C2)C=NN(C#N)[NH+]1C1=CC=C(C)C=C1.[Cl-]
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26
WGK Germany 
3
8
HS Code 
2933998090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
94498
Product name
5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride
Purity
≥85% (HPLC)
Packaging
10MG
Price
$50.6
Updated
2025/07/31
Sigma-Aldrich
Product number
94498
Product name
5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride
Purity
≥85% (HPLC)
Packaging
50MG
Price
$175
Updated
2025/07/31
Cayman Chemical
Product number
15926
Product name
5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride)
Packaging
10mg
Price
$32
Updated
2024/03/01
Cayman Chemical
Product number
15926
Product name
5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride)
Packaging
25mg
Price
$60
Updated
2024/03/01
Cayman Chemical
Product number
15926
Product name
5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride)
Packaging
50mg
Price
$109
Updated
2024/03/01
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CTC Chemical Properties,Usage,Production

Description

5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride) is a redox-sensitive tetrazolium salt used primarily to detect metabolic activity in microorganisms. This membrane-permeable indicator is readily taken up by living cells and reduced by respiratory activity to produce a red fluorescent, water-insoluble formazan crystal (absorption maximum at 450 nm). While the oxidized salt is non-fluorescent, the reduced formazan can be evaluated using a standard rhodamine filter set (excitation: 510-560 nm; emission > 590 nm), although optimum excitation requires a blue 420 nm excitation filter. For flow cytometry, a 480 nm excitation laser combined with detection in the red region is suitable.

Uses

Redox dye producing a fluorescent formazan (only in solid state) upon reduction. It is used as cellular indicator of respiratory activity in cells, especiall for tracking P450 activity.

Uses

5-Cyano-2,3-di-p-tolyltetrazolium Chloride is a monotetrazolium redox dye that is used as a vital stain for actively respiring bacteria. Redox dye producing a fluorescent formazan (only in solid state) upon reduction; used as cellular indicator of respiratory activity in cells.

References

[1] G G RODRIGUEZ. Use of a fluorescent redox probe for direct visualization of actively respiring bacteria.[J]. Applied and Environmental Microbiology, 1992, 58 6: 1801-1808. DOI: 10.1128/aem.58.6.1801-1808.1992
[2] G. CARUSO  E. C  M Mancuso. Combined fluorescent antibody assay and viability staining for the assessment of the physiological states of Escherichia coli in seawaters[J]. Journal of Applied Microbiology, 2003, 95 2: 225-233. DOI: 10.1046/j.1365-2672.2003.01967.x
[3] M E SIERACKI  J N  T L Cucci. Flow cytometric analysis of 5-cyano-2,3-ditolyl tetrazolium chloride activity of marine bacterioplankton in dilution cultures.[J]. Applied and Environmental Microbiology, 1999, 65 6: 2409-2417. DOI: 10.1128/aem.65.6.2409-2417.1999
[4] VISHVESH K BHUPATHIRAJU . Application of a tetrazolium dye as an indicator of viability in anaerobic bacteria[J]. Journal of microbiological methods, 1999, 37 3: Pages 231-243. DOI: 10.1016/s0167-7012(99)00069-x

CTC Preparation Products And Raw materials

Raw materials

Preparation Products

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CTC Suppliers

Aladdin Scientific
Tel
Email
tp@aladdinsci.com
Country
United States
ProdList
57505
Advantage
58
Waterstone Technology, LLC
Tel
--
Fax
--
Email
sales@waterstonetech.com
Country
United States
ProdList
6786
Advantage
30
Cayman Chemical Company
Tel
--
Fax
--
Email
cayman@caymanchem.com
Country
United States
ProdList
6213
Advantage
81
Riedel-de Haen AG
Tel
--
Fax
--
Country
United States
ProdList
6773
Advantage
87
INDOFINE Chemical Company, Inc.
Tel
--
Fax
--
Email
chemical@indofinechemical.com
Country
United States
ProdList
6176
Advantage
69
Research Organics Inc.
Tel
--
Fax
--
Email
info@resorg.com
Country
United States
ProdList
1324
Advantage
72
Polysciences, Inc.
Tel
--
Fax
--
Email
info@polysciences.com
Country
United States
ProdList
1118
Advantage
81

90217-02-0, CTCRelated Search:


  • 5-Cyano-2,3-bis(4-methylphenyl)tetrazolium chloride
  • CTC
  • CYANODITOLYL TETRAZOLIUM CHLORIDE
  • CTC 5-Cyano-2,3-ditolyl tetrazolium chloride
  • 5-CYANO-2,3-DI-(P-TOLYL)TETRAZOLIUM CHLORIDE
  • 5-CYANO-2,3-DITOLYL TETRAZOLIUM CHLORIDE
  • 5-CYANO-2,3-BIS(4-METHYLPHENYL)-2H-TETRAZOLIUM CHLORIDE
  • 2h-tetrazolium, 5-cyano-2,3-bis(4methylphenyl)-, Chloride
  • Cyanotolyl Tetrazolium, Ctc
  • Ctc (biological Stain)
  • CYANOTOLYL TETRAZOLIUM CHLORIDE (CTC)
  • 5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride, ≥85% (HPLC)
  • 5-Cyano-2,3-ditolyl-2H-tetrazolium chloride
  • 5-Cyano-2,3-di-p-tolyl-2H-tetrazol-3-ium Chloride
  • 90217-02-0
  • C16H14ClN5
  • C16H14N5Cl
  • BioChemical
  • Biochemicals and Reagents
  • Stains and Dyes
  • Luminescent Compounds/Detection
  • marker
  • Tetrazolium salt