PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1
- Product Name
- PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1
- CAS No.
- 136112-00-0
- Chemical Name
- PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1
- Synonyms
- PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1;PHENOL:CHLOROFORM:ISOAMYLALCOHOL 49.5:49 .5:1;phenol - chloroform - isoamyl alcohol mixture;PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 USP/EP/BP;Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized;PHENOL/CHLOROFORM/ISOAMYL ALCOHOL (25:24:1), STABILZED,;PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1, FOR MOL. BIOLOGY;Phenol mixt. with 3-methyl-1-butanol and trichloromethane;PHENOL:CHLOROFORM:ISOAMYL ALC. 125:24:1, FOR MOL. BIOLOGY;Phenol:Chloroform:Isoamyl Alcohol (25:24:1) pH 8.0 for molecular biology
- CBNumber
- CB4502825
- Molecular Formula
- C12H19Cl3O2
- Formula Weight
- 301.63706
- MOL File
- 136112-00-0.mol
PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 Property
- Density
- 1.29 g/mL at 20 °C
- Flash point:
- 79 °C
- storage temp.
- 2-8°C
- form
- Liquid
- PH
- 7.7-8.3(H2O-phase, after extraction with H2O, 1:1)
- Water Solubility
- Soluble in water.
- λmax
- λ: 330 nm Amax: 0.2
λ: 405 nm Amax: 0.1
λ: 510 nm Amax: 0.1 - InChI
- InChI=1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H
- InChIKey
- ZYWFEOZQIUMEGL-UHFFFAOYSA-N
- SMILES
- ClC([H])(Cl)Cl.O([H])C([H])([H])C([H])([H])C([H])(C([H])([H])[H])C([H])([H])[H].O([H])C1C([H])=C([H])C([H])=C([H])C=1[H]
- Absorption
- cut-off at 360nm
Safety
- Hazard Codes
- T
- Risk Statements
- 23/24/25-34-40-48/20/21/22-68-63
- Safety Statements
- 26-36/37/39-45
- RIDADR
- UN 2810 6.1/PG 2
- WGK Germany
- 3
- F
- 8-10-23
- HazardClass
- 8
- HS Code
- 29420000
- Storage Class
- 6.1A - Combustible acute toxic Cat. 1 and 2
very toxic hazardous materials - Hazard Classifications
- Acute Tox. 3 Oral
Acute Tox. 4 Dermal
Acute Tox. 4 Inhalation
Aquatic Chronic 2
Carc. 2
Eye Dam. 1
Muta. 2
Repr. 2
Skin Corr. 1B
STOT RE 1 Oral
STOT RE 2
STOT SE 3
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Danger
- Hazard statements
-
H314Causes severe skin burns and eye damage
H341Suspected of causing genetic defects
H351Suspected of causing cancer
H372Causes damage to organs through prolonged or repeated exposure
H373May cause damage to organs through prolonged or repeated exposure
H411Toxic to aquatic life with long lasting effects
- Precautionary statements
-
P202Do not handle until all safety precautions have been read and understood.
P273Avoid release to the environment.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- 77617
- Product name
- Phenol – chloroform – isoamyl alcohol mixture
- Purity
- BioUltra, for molecular biology, 25:24:1
- Packaging
- 100ml
- Price
- $130
- Updated
- 2025/07/31
- Product number
- 77617
- Product name
- Phenol – chloroform – isoamyl alcohol mixture
- Purity
- BioUltra, for molecular biology, 25:24:1
- Packaging
- 500ml
- Price
- $416
- Updated
- 2025/07/31
- Product number
- 77619
- Product name
- Phenol – chloroform – isoamyl alcohol mixture
- Purity
- BioUltra, for molecular biology, 125:24:1
- Packaging
- 100ml
- Price
- $113
- Updated
- 2025/07/31
- Product number
- 77619
- Product name
- Phenol – chloroform – isoamyl alcohol mixture
- Purity
- BioUltra, for molecular biology, 125:24:1
- Packaging
- 500ml
- Price
- $364.45
- Updated
- 2025/07/31
- Product number
- 77618
- Product name
- Phenol – chloroform – isoamyl alcohol mixture
- Purity
- BioUltra, for molecular biology, 49.5:49.5:1
- Packaging
- 100ml
- Price
- $102
- Updated
- 2025/07/31
PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 Chemical Properties,Usage,Production
Chemical Properties
clear yellow solution
Uses
Phenol:Chloroform:Isoamyl alcohol 25:24:1 is used in the extraction of DNA for the generation of DNA minisatellite fingerprints. It is also used for purifying RNA.
Biological Activity
When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.
Synthesis
Examples of the application of phenol-chloroform-isoamyl alcohol mixture are as follows:
(1) For the extraction of genomic DNA of a glycoconjugate-producing bacterium suitable for whole genome sequencing, extraction buffer and lysozyme were added to a culture of glycoconjugate-producing bacterium, which was left to stand for 10 min at 37??C, then proteinase K was added, and left to stand for 1 h at 65??C; a mixture of phenol-chloroform-isoamyl alcohol was added, and extracted; a mixture of chloroform-isoamyl alcohol was added, and extracted; cold ethanol and sodium acetate were added, and the precipitate was washed by ethanol and dried at room temperature, and RNA was added to dissolve it. centrifugation, the precipitate was washed with ethanol and dried at room temperature, and solubilized by adding DNA extraction buffer; add RNAase, and let it stand for 10 min at 37??; add PEG6000, mix well, and let it stand for 10 min at 50??; add a mixed solution of phenol-chloroform-isoamyl alcohol, and extract; add a mixed solution of chloroform-isoamyl alcohol, and extract; add a solution of cold ethanol and sodium acetate, and centrifugation, the precipitate was washed with ethanol and dried at room temperature, and the precipitate was washed with ethanol. After drying at room temperature, add ddH2O or TE buffer to dissolve, which is the extracted DNA solution.
PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 manufacturers
- Product
- PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 136112-00-0
- Price
- US $1.10/g
- Min. Order
- 1g
- Purity
- 99.9%
- Supply Ability
- 100 Tons min
- Release date
- 2021-07-22