4-Chloro-7-nitrobenzofurazan

ChemicalBook > CAS DataBase List > 4-Chloro-7-nitrobenzofurazan

4-Chloro-7-nitrobenzofurazan

Product Name: 4-Chloro-7-nitrobenzofurazan
CAS No.: 10199-89-0
Chemical Name: 4-Chloro-7-nitrobenzofurazan
Synonyms: NBD-CL;4-chloro-7-nitrobenzo[c][1,2,5]oxadiazole;4-Chloro-7-nitro-1,2,3-benzoxadiazole;4-CHLORO-7-NITROBENZOFURAZAN;NBD CHLORIDE;4-chloro-7-nitrobenzo-2,1,3-oxadiazole;7-CHLORO-4-NITROBENZO-2-OXA-1,3-DIAZOLE;4-CHLORO-7-NITROBENZOFURAZAN, FOR FLUORE SCENCE;NBC-1;nbd-c1
CBNumber: CB5173637
Molecular Formula: C6H2ClN3O3
Formula Weight: 199.55
MOL File: 10199-89-0.mol

Less

4-Chloro-7-nitrobenzofurazan Property

Melting point:: 97-99 °C(lit.)
Boiling point:: 333.1±45.0 °C(Predicted)
Density: 2.0589 (rough estimate)
refractive index: 1.5810 (estimate)
storage temp.: Inert atmosphere,Room Temperature
solubility: Soluble in methanol, dimethylsulfoxide, dimethylformamide and chloroform.
pka: -5.65±0.50(Predicted)
form: Crystalline Powder
color: Yellow to light brown
Water Solubility: soluble
BRN: 614212
CAS DataBase Reference: 10199-89-0(CAS DataBase Reference)
EPA Substance Registry System: 2,1,3-Benzoxadiazole, 4-chloro-7-nitro- (10199-89-0)

Less

Safety

Hazard Codes: Xn,Xi
Risk Statements: 22-36/38
Safety Statements: 36/37/39
RIDADR: UN 2811 6.1/PG 3
WGK Germany: 3
RTECS: DF8002400
F: 10-21
TSCA: Yes
HS Code: 29349990

Less

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

Less

N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 163260
Product name: 4-Chloro-7-nitrobenzofurazan
Purity: 98%
Packaging: 1g
Price: $51
Updated: 2024/03/01

Sigma-Aldrich

Product number: 163260
Product name: 4-Chloro-7-nitrobenzofurazan
Purity: 98%
Packaging: 5g
Price: $71.3
Updated: 2024/03/01

TCI Chemical

Product number: A5592
Product name: NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole)[for HPLC Labeling]
Purity: >98.0%(T)
Packaging: 1g
Price: $42
Updated: 2024/03/01

TCI Chemical

Product number: A5592
Product name: NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole)[for HPLC Labeling]
Purity: >98.0%(T)
Packaging: 5g
Price: $121
Updated: 2024/03/01

Alfa Aesar

Product number: A14165
Product name: 4-Chloro-7-nitrobenzofurazan, 99%
Packaging: 5g
Price: $65.2
Updated: 2024/03/01

Less

4-Chloro-7-nitrobenzofurazan Chemical Properties,Usage,Production

Chemical Properties

yellow to light brown crystalline powder

Uses

4-Chloro-7-nitrobenzofurazan is used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines. It is utilized in the preparation of fluorescent phospholipid-derivative, hydroxynaphthofurazan and 4-chloro-7-nitrobenzofurazan- didecanoylphosphatidylethanolamine. It serves as a fluorescent reagent to label free sulfhydryls and N-terminals within proteins and trapping agent for cysteine sulfenic acid. It is a sensitive chromogenic and fluorogenic reagent. It reacts with trans-1-methoxy-3-(trimethylsilyloxy)-1,3-butadiene (Danishefsky's diene) to prepare regioselectively the silyl enol ether. Further, it is used to label peptides, proteins and other biomolecules. In addition it is used in the synthesis of fluorescent phospholipid-derivative, NBD-didecanoylphosphatidylethanolamine, functionalized hydroxynaphthofurazan and 7-nitrobenzofurazan (NBD)-labeled maleimide.

Uses

4-Chloro-7-nitrobenzofurazan is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. 4-Chloro-7-nitrobenzofurazan has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.

Definition

ChEBI: A benzoxadiazole that is 2,1,3-benzoxadiazole which is substituted at position 4 by chlorine and at position 7 by a nitro group.

General Description

4-Chloro-7-nitrobenzofurazan (NBD-Cl) is a highly sensitive chromogenic and fluorogenic reagent. It is reported to react spontaneously with trans-1-methoxy-3-(trimethylsilyloxy)-1,3-butadiene (Danishefsky′s diene), to afford regioselectively the silyl enol ether, via normal electron-demand Diels-Alder (NEDDA) reaction. A study of the molecular structure, vibrational spectra and NBO (Natural Bond Orbital) analysis of NBD–Cl has been undertaken. Its utility as a pre-column derivatization agent has been examined.

in vitro

4-Chloro-7-nitrobenzofurazan(NBD chloride) forms highly fluorescent derivatives for detection of all the protein amino acids. In addition, NBD-Cl provides a simple and sensitive method for determination of N-terminal amino acids. The differences in intensity and color of fluorescence can be used to advantage to identify prolyl peptides.

Purification Methods

Wash the solid with H2O, and it recrystallises from aqueous EtOH (1:1) as pale yellow needles. It sublimes in a vacuum [Gosh & Whitehouse Biochem J 108 155 1968, UV, NMR: Bolton et al. J Chem Soc 1004 1966].

4-Chloro-7-nitrobenzofurazan Preparation Products And Raw materials

Raw materials

Preparation Products

Less

4-Chloro-7-nitrobenzofurazan Suppliers

Americas 1 Austria 1 Belgium 1 Canada 2 China 155 Europe 3 Germany 5 India 5 Japan 3 South Korea 1 Switzerland 3 Ukraine 1 United Kingdom 10 United States 32 Global 223

Energy Chemical

Tel: 021-021-58432009 400-005-6266
Fax: 021-58436166
Email: sales8178@energy-chemical.com
Country: China
ProdList: 44751
Advantage: 61

Anhui Hehu Biological Technology Co., Ltd.

Tel: 153-75048740 15375048740
Email: 2328767661@qq.com
Country: China
ProdList: 493
Advantage: 58

J & K SCIENTIFIC LTD.

Tel: 010-82848833 400-666-7788
Fax: 86-10-82849933
Email: jkinfo@jkchemical.com
Country: China
ProdList: 94838
Advantage: 76

Meryer (Shanghai) Chemical Technology Co., Ltd.

Tel: 4006608290; 18621169109
Fax: 86-21-61259102
Email: market03@meryer.com
Country: China
ProdList: 40241
Advantage: 62

Alfa Aesar

Tel: 400-6106006
Fax: 021-67582001/03/05
Email: saleschina@alfa-asia.com
Country: China
ProdList: 30132
Advantage: 84

TCI (Shanghai) Development Co., Ltd.

Tel: 021-67121386
Fax: 021-67121385
Email: Sales-CN@TCIchemicals.com
Country: China
ProdList: 24539
Advantage: 81

Beijing Ouhe Technology Co., Ltd

Tel: 010-82967028 13552068683
Fax: +86-10-82967029
Email: 2355560935@qq.com
Country: China
ProdList: 12458
Advantage: 60

Shanghai Longsheng chemical Co.,Ltd.

Tel: 021-58099652-8005 13585536065
Fax: 021-58099609
Email: bin.wu@shlschem.com
Country: China
ProdList: 9816
Advantage: 59

Shanghai Hanhong Scientific Co.,Ltd.

Tel: 021-54306202 13764082696;
Email: info@hanhongsci.com
Country: China
ProdList: 42982
Advantage: 64

Chemsky (shanghai) International Co.,Ltd

Tel: 021-50135380
Email: shchemsky@sina.com
Country: China
ProdList: 15421
Advantage: 60

Syntechem Co.,Ltd

Tel
Fax: E-Mail Inquiry
Email: info@syntechem.com
Country: China
ProdList: 12990
Advantage: 57

BEST-REAGENT

Tel: 400-1166-196 18981987031
Fax: 028-84555506 800101999
Email: cdhxsj@163.com
Country: China
ProdList: 11726
Advantage: 57

skychemical Co.Ltd

Tel: 021-20958197 20965099
Fax: 021-20960837
Email: sales@skychemical.com
Country: China
ProdList: 1184
Advantage: 57

RYSS TECH LTD

Tel: 400-188-0725 +86 21 34310725 13611771617
Fax: +86 21 34311076
Country: China
ProdList: 2002
Advantage: 57

Tianjin heowns Biochemical Technology Co., Ltd.

Tel: 400 638 7771
Email: sales@heowns.com
Country: China
ProdList: 14443
Advantage: 57

Hunan Hui Bai Shi Biotechnology Co., Ltd.

Tel: 0731-85526065 13308475853
Email: ivy@hnhbsj.com
Country: China
ProdList: 4550
Advantage: 62

Spectrum Chemical Manufacturing Corp.

Tel: 021-021-021-67601398-809-809-809 15221380277
Fax: 021-57711696
Email: marketing_china@spectrumchemical.com
Country: China
ProdList: 9664
Advantage: 60

Hangzhou Sage Chemical Co., Ltd.

Tel: +86057186818502 13588463833
Fax: Pls mail us for more information!
Email: info@sagechem.com
Country: China
ProdList: 10269
Advantage: 58

Shanghai civi chemical technology co.,Ltd

Tel: 86-21-34053660
Fax: 86-21-34053661
Email: sale@labgogo.com
Country: China
ProdList: 9872
Advantage: 52

Thermo Fisher Scientific

Tel: 800-810-5118
Fax: +86-10-84193589
Email: cnchemical@thermofisher.com
Country: China
ProdList: 17779
Advantage: 75

Beijing HuaMeiHuLiBiological Chemical

Tel: 010-56205725
Fax: 010-65763397
Email: waley188@sohu.com
Country: China
ProdList: 12338
Advantage: 58

Shanghai Topbiochem Technology Co., Ltd

Tel: 021-58170097
Email: info@topbiochem.com
Country: China
ProdList: 9439
Advantage: 58

9ding chemical ( Shanghai) Limited

Tel: 4009209199
Fax: 86-021-52271987
Email: sales@9dingchem.com
Country: China
ProdList: 22519
Advantage: 55

UHN Shanghai Research & Development Co., Ltd.

Tel: 021-58958002 18930822973
Fax: +86 (21) 5895-8628
Email: SALES@UHNSHANGHAI.COM
Country: China
ProdList: 977
Advantage: 58

Shanghai Aladdin Bio-Chem Technology Co.,LTD

Tel: 18521735133 18521732826;
Fax: 021-50323701
Email: market@aladdin-e.com
Country: China
ProdList: 25015
Advantage: 65

The future of Shanghai Industrial Co., Ltd.

Tel: 021-61552785
Fax: 021-55660885
Email: sales@shshiji.com
Country: China
ProdList: 9552
Advantage: 55

Shanghai Raise Chemical Technology Co.,Ltd

Tel: +86-021-50935922
Fax: +86-021-33847795
Country: China
ProdList: 7876
Advantage: 55

Chengdu AstaTech Trading Co., Ltd./AstaTech (Chengdu) Pharma. Co., Ltd.

Tel: +86-28-85122536 85324413
Fax: +86-28-85326443
Email: market@astatech.cn
Country: China
ProdList: 8033
Advantage: 55

TargetMol Chemicals Inc.

Tel: 021-33632979 15002134094
Fax: 021-33632979
Email: marketing@targetmol.com
Country: China
ProdList: 7934
Advantage: 58

Jinan Mingya Medical Technology Co., Ltd.

Tel: 0531-85828981
Fax: +86-531-85806006
Email: 864598798@qq.com
Country: China
ProdList: 588
Advantage: 58

Aloespharm Co., Ltd.

Tel: 021-20960837
Fax: 021-20960837
Email: market@aloespharm.com
Country: China
ProdList: 1303
Advantage: 55

Shanghai CanSpecsci Instrument Co., Ltd.

Tel: 400-6087598 15021221957
Fax: 4006087598-8012
Email: order@canspecsci.com
Country: China
ProdList: 2071
Advantage: 56

Shanghai Macklin Biochemical Co.,Ltd.

Tel: 15221275939 15221275939
Fax: 021-50706099
Email: shenlinxing@macklin.cn
Country: China
ProdList: 15878
Advantage: 55

Sigma-Aldrich

Tel: 021-61415566 800-8193336
Email: orderCN@merckgroup.com
Country: China
ProdList: 51471
Advantage: 80

Alta Scientific Co., Ltd.

Tel: 022-6537-8550 15522853686
Fax: 022-2532-9655
Email: sales@altasci.com.cn
Country: China
ProdList: 4515
Advantage: 55

Chengdu RunZeBenTu Chemical Co., Ltd

Tel: 028-88469284 18000562381
Email: rzbtsj@163.com
Country: China
ProdList: 9958
Advantage: 56

skychemical Co.Ltd

Tel: 021-20960837,QQ1332204249
Fax: 021-20960837
Email: sales@skychemical.com
Country: China
ProdList: 3317
Advantage: 55

ATK CHEMICAL COMPANY LIMITED

Tel: 3429815786 13301662590
Fax: +86-21-51619052
Email: sales@atkchemical.com
Country: China
ProdList: 10006
Advantage: 55

Henan CoreyChem Co., Ltd

Tel: 0371-86658258
Fax: 0371-60996044
Email: info@coreychem.com
Country: China
ProdList: 10457
Advantage: 58

Shanghai EFE Biological Technology Co., Ltd.

Tel: 021-65675885 18964387627
Fax: 021-65675885
Email: info@efebio.com
Country: China
ProdList: 9709
Advantage: 58

Shanghai Xilong Biochemical Technology Co., Ltd.

Tel: 021-52907766-8042
Fax: 021-52906523
Country: China
ProdList: 9947
Advantage: 58

Chengdu HuaNa Chemicals Co., Ltd.

Tel: 028-86612776; 13699069380
Fax: -
Email: 515238037@qq.com
Country: China
ProdList: 963
Advantage: 55

Shanghai YuanYe Biotechnology Co., Ltd.

Tel: 021-61312847; 18021002903
Fax: QQ:3008007432
Email: 3008007409@qq.com
Country: China
ProdList: 27322
Advantage: 60

Shandong Xiya Chemical Co., Ltd.

Tel: 4009903999 13395398332
Fax: 0539-6365991
Email: sales@xiyashiji.com
Country: China
ProdList: 20810
Advantage: 60

Grader reagent

Tel: 18221735425
Email: sales@xinpingchem.com
Country: China
ProdList: 9951
Advantage: 58

Wuxi Asia Peptide Biotechnology Limited Company

Tel: 0510-83583050 13771062561
Fax: 0510-83583039
Email: willsun@peptide-search.com
Country: China
ProdList: 6716
Advantage: 58

Beijing Solarbio Science & Tecnology Co., Ltd.

Tel: 010-50973130 4009686088
Email: 3193328036@qq.com
Country: China
ProdList: 29797
Advantage: 68

Changchun BC＆HC Pharmaceutical Technology Co.,Ltd

Tel: 0431-0431-80871588 15804318257
Fax: 0431-88417879
Email: 3468242165@qq.com
Country: China
ProdList: 195
Advantage: 58

Amadis Chemical Company Limited

Tel: 571-89925085
Fax: 0086-571-89925065
Email: sales@amadischem.com
Country: China
ProdList: 131981
Advantage: 58

Lynnchem

Tel: 86-(0)29-85992781 17792393971
Email: info@lynnchem.com
Country: China
ProdList: 4587
Advantage: 58

Less

View Lastest Price from 4-Chloro-7-nitrobenzofurazan manufacturers

Hebei Mojin Biotechnology Co., Ltd

Product: 4-Chloro-7-nitrobenzofurazan 10199-89-0
Price: US $0.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 50000KG/month
Release date: 2023-09-06

Hebei Guanlang Biotechnology Co., Ltd.

Product: 4-Chloro-7-nitrobenzofurazan 10199-89-0
Price: US $10.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 10 mt
Release date: 2023-01-10

Career Henan Chemical Co

Product: 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole 10199-89-0
Price: US $2.00/kg
Min. Order: 1kg
Purity: 99%
Supply Ability: ask
Release date: 2018-12-22

10199-89-0, 4-Chloro-7-nitrobenzofurazanRelated Search:

4-Chloro-7-nitrobenzofurazan Nitrobenzene BENZOFURAZAN N-BENZYL-4-NITRO-2,1,3-BENZOXADIAZOL-5-AMINE 7-CHLORO-4-NITRO-5-PYRROLIDIN-1-YL-BENZO[1,2,5]OXADIAZOLE 7-CHLORO-4-NITRO-5-PIPERIDINO-2,1,3-BENZOXADIAZOLE 5-AMINO-7-CHLORO-4-NITROBENZOFURAZAN 7-CHLORO-N,N-DIETHYL-4-NITRO-2,1,3-BENZOXADIAZOL-5-AMINE 2-(7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YLAMINO)-ETHANOL 3-(7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YLAMINO)-PROPAN-1-OL AKOS BB-7022 7-CHLORO-5-(4-METHYL-PIPERAZIN-1-YL)-4-NITRO-BENZO[1,2,5]OXADIAZOLE 7-CHLORO-5-MORPHOLIN-4-YL-4-NITRO-BENZO[1,2,5]OXADIAZOLE (7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YL)-PYRIDIN-2-YL-AMINE N-[4-(7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YLAMINO)-PHENYL]-ACETAMIDE TIMTEC-BB SBB005650 (7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YL)-DIMETHYL-AMINE 4-CHLORO-5,7-DINITROBENZOFURAZAN

4-CHLORO-7-NITROBENZOFURAZAN, FOR FLUORE SCENCE

7-CHLORO-4-NITROBENZ-2-OXA-1-3-DIAZOLECR YSTALLINE

4-Chloro-7-nitrobenzo-2,1,3-oxadiazole~NBDchloride

2,1,3-Benzoxadiazole, 4-chloro-7-nitro-

4-chloro-7-nitrobenzo-2,1,3-oxadiazole

NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]

4-chloro-7-nitrobenzofurazane

4-Chloro-7-nitro-1,2,3-benzoxadiazole, NBD-chloride

NBC-1

NBD chloride,98%

NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole)

4-Chloro-7-nitro-1,2

4-chloro-7-nitrobenzo[c][1,2,5]oxadiazole

7-Chloro-4-nitro-2,1,3-benzoxadiazole

NSC 228140

4-Chloro-7-nitrobenzofurazan 4-Chloro-7-nitro-2,1,3-benzoxadiazole

4-Chloro-7-nitro-2,1,3-benzoxadiazole 99%

4-chloro-7-nitro-benzofuraza

4-nitro-7-chlorobenzofurazan

nbd-c1

7-CHLORO-4-NITRO-2,1,3-BENZOXADIAZOL

7-CHLORO-4-NITROBENZO-2-OXA-1,3-DIAZOLE

7-CHLORO-4-NITROBENZOFURAZAN

AKOS BC-0686

4-CHLORO-7-NITRO-2,1,3-BENZOXADIAZOLE

4-CHLORO-7-NITROBENZ-2-OXA-1,3-DIAZOLE

4-CHLORO-7-NITRO-BENZO[1,2,5]OXADIAZOLE

4-CHLORO-7-NITROBENZO-2-OXA-1,3-DIAZOLE

4-CHLORO-7-NITROBENZOFURAZAN

4-CHLORO-7-NITROBENZUFURAZAN

4-Chloro-7-nitro-1,2,3-benzoxadiazole (NBD-Cl )

4-Chloro-7-nitrobenzo-2-furazan, NBF-Cl

1-Chloro-4-nitrobenzoxadiazole

LABOTEST-BB LT00238516

NBD CHLORIDE

NBD-CL

TIMTEC-BB SBB005345

4-Chloro-7-nitro-1,2,3-benzoxadiazole

NBD-Cl [4-Chloro-7-nitrobenzofurazan] *CAS 10199-89-0*

7-CHLORO-4-NITROBENZ-2-OXA-1,3-DIAZOLE

Benzofurazan, 4-chloro-7-nitro-

Shokat-update-comp17

NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole)[for HPLC Labeling]&gt

NBD-Cl [4-Chloro-7-nitrobenzofurazan

oro-7-nitrobenzo-2-oxa-1,3-diazoL

4-Chloro-7-nitro-2,1,3-benzoxadiazole(NBD-Cl)

4-Choro-7-nitrobenzofurazan

4-Chloro-7-nitrobenzo-2-furazan

10199-89-0

C6H2ClN3O3

Mercapto Group Labeling Reagents for Fluorescence HPLC

HPLC Labeling Reagents

Fluorescence Detection (HPLC Labeling Reagents)

Amino Group Labeling Reagents for Fluorescence HPLC

Functional Group Reactive Labels

Functional Group Reactive Labels for Immunoassays

Fluorescent Probes, Labels, Particles and Stains

Detection