2-([1-(3-[[3-(DIMETHYL(3-[4-[(E)-(3-METHYL-1,3-BENZOXAZOL-3-IUM-2-YL)METHYLIDENE]-1(4H)-QUINOLINYL]PROPYL)AMMONIO)PROPYL](DIMETHYL)AMMONIO]PROPYL)-4(1H)-QUINOLINYLIDENE]METHYL)-3-METHYL-1,3-BENZOXAZOL-3-IUM TETRAIODIDE

2-([1-(3-[[3-(DIMETHYL(3-[4-[(E)-(3-METHYL-1,3-BENZOXAZOL-3-IUM-2-YL)METHYLIDENE]-1(4H)-QUINOLINYL]PROPYL)AMMONIO)PROPYL](DIMETHYL)AMMONIO]PROPYL)-4(1H)-QUINOLINYLIDENE]METHYL)-3-METHYL-1,3-BENZOXAZOL-3-IUM TETRAIODIDE Property

Melting point:

>250 ℃

solubility 

Soluble in dimethyl sulfoxide, water

form 

powder

color 

Orange-red

Appearance

orange solution

λmax

491 nm

ex/em

491/509 nm

ε(extinction coefficient)

98900 cm−1M−1

Φ(quantum yield)

0.52

Biological Applications

Nucleic acid hybridization; nucleic acid sequencing; detecting nucleic acids,cells,human papilloma virus (HPV),pathogens,spores,cancer cells,stress biomarkers,viruses

Hazard and Precautionary Statements (GHS)

2-([1-(3-[[3-(DIMETHYL(3-[4-[(E)-(3-METHYL-1,3-BENZOXAZOL-3-IUM-2-YL)METHYLIDENE]-1(4H)-QUINOLINYL]PROPYL)AMMONIO)PROPYL](DIMETHYL)AMMONIO]PROPYL)-4(1H)-QUINOLINYLIDENE]METHYL)-3-METHYL-1,3-BENZOXAZOL-3-IUM TETRAIODIDE Chemical Properties,Usage,Production

Description

YODi-1 (Oxazole Yellow Homodimer, also known as YOYO^®-1) is a green fluorescent carbocyanine dimeric dye. YODi-1 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits over a thousand-fold fluorescence enhancement upon binding to dsDNA.

The bright fluorescence signal and low background make YODi-1 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments. YODi-1 can also be used for the analysis of single molecules of DNA.

Chemical Properties

ex/em: 491/509 nm
Extinction coefficient (ε): 98900 cm??1M??1
Quantum yield (Φ): 0.52

Uses

Thiazole orange dimer YOYO 1 is a green fluorescent dye used for DNA staining. It belongs to the monomethine cyanine dye family and is a tetracationic homodimer of oxazole yellow (abbreviated as YO, hence the name YOYO), usually provided as a tetraiodide salt. In aqueous buffer, the free YOYO-1 dye (λmax 458 nm; λmax 564 nm) has a very low fluorescence quantum yield, but after binding to double-stranded DNA through diintercalation, the fluorescence intensity is increased by 3200 times (λmax 489 nm; λmax 509 nm).

References

[1] Wang L, et al.Ultrafast Transient Absorption Spectra of Photoexcited YOYO-1 molecules call for additional investigations of their fluorescence quenching mechanism. J Photochem Photobiol A Chem. 2018 Dec 1;367:411-419.

Abs/Em Maxima

490/508 nm

Fluoresene quantum yield

0.37