YO-PRO 1

YO-PRO 1 Property

Melting point:

>250 ℃

storage temp. 

2-8°C

solubility 

H2O: soluble5, clear (warmed)

form 

powder

color 

yellow to orange-brown

Appearance

Green solution

biological source

r at blood

Water Solubility 

H2O: 5, clear (warmed)

λmax

491 nm

ex/em

491/509 nm

ε(extinction coefficient)

52000 cm−1M−1

Φ(quantum yield)

0.44

Biological Applications

Nucleic acid hybridization; nucleic acid sequencing; detecting nucleic acids,cells,bacteria,immunodeficiency virus,microorganisms; counting embryoblasts,viruses; apoptosis assay; white blood cell functional assay

Safety

RIDADR 

UN 2811 6.1 / PGIII

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H301Toxic if swalloed

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

YO-PRO 1 Chemical Properties,Usage,Production

Description

YO-TAP-1 (Oxazole Yellow Monomer, also known as YO-PRO^®-1) is a green fluorescent carbocyanine monomeric dye. YO-TAP-1 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a significant fluorescence enhancement upon binding to dsDNA.

YO-TAP-1 has a bright fluorescence signal and low background. The dye is useful for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in microscopy. It may be used for monitoring of cell viability and apoptotic and dead cell detection in culture.

Chemical Properties

Appearance: Green solution
ex/em: 491/509 nm
Extinction coefficient (ε): 52000 cm??1M??1
Quantum yield (Φ): 0.44

Uses

Hybridisation and cloning studies

Definition

ChEBI: An unsymmetrical C1 cyanine dye having 1,3-benzoxazol-2-yl and quinolinium-4-yl substituents.

Biochem/physiol Actions

Oxazole yellow is a fluorescent cyanine dye that binds to DNA and is used to detect apoptotic cells. Oxazole yellow does not enter live cells. However, during apoptosis the cytoplasmic membrane becomes slightly permeable, allowing entry of the dye. Oxazole yellow is often used along with propidium iodide (catalog no. P4170), a DNA dead cell stain that does not enter either live or apoptotic cells. The λEx/λEm of Oxazole yellow is 491/509.

storage

-20°C (protect from light)

Abs/Em Maxima

491/508 nm