Sulforhodamine B
- Product Name
- Sulforhodamine B
- CAS No.
- 3520-42-1
- Chemical Name
- Sulforhodamine B
- Synonyms
- ACID RED 52;ACID RED;CI 45100;LISSAMINE RHODAMINE B;red106;AKA106;XYLENE RED;KITON RED S;LISSAMINE RHODAMINE;redno.106
- CBNumber
- CB6223683
- Molecular Formula
- C27H31N2NaO7S2
- Formula Weight
- 582.66
- MOL File
- 3520-42-1.mol
Sulforhodamine B Property
- Density
- 1.472[at 20℃]
- storage temp.
- room temp
- solubility
- methanol: 1 mg/mL
- Colour Index
- 45100
- form
- powder
- color
- Brown to Very Dark Purple
- Water Solubility
- 95.3g/L at 20℃
- BRN
- 3886008
- InChI
- InChI=1S/C27H30N2O7S2.Na.H/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);;
- InChIKey
- XCRMYPBRGZRYEU-UHFFFAOYSA-N
- SMILES
- S(C1C=C(S(O)(=O)=O)C=CC=1C1C2=CC=C(N(CC)CC)C=C2[O+]=C2C=C(N(CC)CC)C=CC=12)([O-])(=O)=O.[NaH]
- LogP
- -2.2 at 20℃
- EPA Substance Registry System
- C.I. Acid Red 52 (3520-42-1)
Safety
- Hazard Codes
- Xi
- Risk Statements
- 36/37/38
- Safety Statements
- 22-24/25-26-36/37/39
- WGK Germany
- 2
- RTECS
- BP6750000
- F
- 8
- TSCA
- Yes
- HS Code
- 32129000
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
- Precautionary statements
-
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P321Specific treatment (see … on this label).
P332+P313IF SKIN irritation occurs: Get medical advice/attention.
P362Take off contaminated clothing and wash before reuse.
N-Bromosuccinimide Price
- Product number
- 230162
- Product name
- Sulforhodamine B
- Purity
- Dye content 75 %
- Packaging
- 1g
- Price
- $38.8
- Updated
- 2024/03/01
- Product number
- 230162
- Product name
- Sulforhodamine B
- Purity
- Dye content 75 %
- Packaging
- 5g
- Price
- $120
- Updated
- 2024/03/01
- Product number
- F0143
- Product name
- Food Red No. 106
- Packaging
- 25g
- Price
- $127
- Updated
- 2024/03/01
- Product number
- A0600
- Product name
- Acid Red 52
- Packaging
- 25g
- Price
- $81
- Updated
- 2024/03/01
- Product number
- A14769
- Product name
- Sulforhodamine B sodium salt
- Packaging
- 5g
- Price
- $49.8
- Updated
- 2024/03/01
Sulforhodamine B Chemical Properties,Usage,Production
Chemical Properties
This substance is a deep red powder. It is easily soluble in water, forming a bluish-red solution with yellow fluorescence. When sodium hydroxide solution is added, it also appears bluish-red. Additionally, it is soluble in ethanol. In concentrated sulfuric acid, it turns orange-yellow and red after dilution.
Uses
Sulforhodamine B sodium salt has been used:
- for relative total cell mass quantification
- to investigate the viscosity of coacervates
- as a molecular rotor for microviscosity determination in aerosol droplets
Uses
Sulforhodamine B Sodium Salt is a rhodamine dye widely used in nonpermanent tattoos. Studies show that Sulforhodamine B can be used as a potential new therapeutic agent for the treatment of the acute respiratory distress syndrome. Dyes and metabolites, Environmental Testing
Application
Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening.
It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts.
It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material.
Preparation
4-Formylbenzene-1,3-disulfonic acid and 3-(Diethylamino)phenol condensation, the products with sulfuric acid dehydration, and ferric chloride oxidation, and finally into sodium salt.
Definition
ChEBI: Sulforhodamine B is an organic sodium salt having 4-[3,6-bis(diethylamino)-2,7-dimethylxanthenium-9-yl]benzene-1,3-disulfonate as the counterion.
General Description
Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination.
Flammability and Explosibility
Not classified
Biochem/physiol Actions
Sulforhodamine B sodium salt is useful to measure cell biomass and also for cytotoxicity screening.
Properties and Applications
bright blue light pink. Dark red light brown powder. Soluble in water, in blue light red, with fluorescence, also soluble in alcohol. The strong sulfuric acid dyes is orange, diluted for red, with sodium hydroxide solution is blue light red. Mainly used for wool, silk and polyamide fiber dyeing, also can be used for wool and a variety of fabric dyeing, still can be in wool and silk fabric printing directly, also can used for leather dyeing.
| Standard | Light Fastness | Soaping | Persperation Fastness | Oxygen bleaching | Fastness to seawater | |||
| Fading | Stain | Fading | Stain | Fading | Stain | |||
| ISO | 3 | 3-4 | 3 | 4 | 1 | 2 | 3 | 2 |
| AATCC | 2-3 | 3 | 3 | 3 | 1 | 2 | 4 | 2-3 |
Sulforhodamine B Preparation Products And Raw materials
Raw materials
Preparation Products
Sulforhodamine B Suppliers
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View Lastest Price from Sulforhodamine B manufacturers
- Product
- SULFORHODAMINE B 3520-42-1
- Price
- US $40.00/kg
- Min. Order
- 1kg
- Purity
- 0.99
- Supply Ability
- 10 tons
- Release date
- 2023-09-08
- Product
- Sulforhodamine B 3520-42-1
- Price
- US $1.00/Kg
- Min. Order
- 1Kg
- Purity
- 98%
- Supply Ability
- 20T
- Release date
- 2024-07-12
- Product
- Sulforhodamine B 3520-42-1
- Price
- US $6.00-1.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- g-kg-tons, free sample is available
- Release date
- 2024-03-28