DAF-FM
- Product Name
- DAF-FM
- CAS No.
- 254109-20-1
- Chemical Name
- DAF-FM
- Synonyms
- DAF-FM;DAF-FM solution;DAF-FM >=98% (HPLC);DIAMINOFLUORESCEIN-FM;PPRXFLLOWKZHMK-UHFFFAOYSA-N;DAF-FM - CAS 254109-20-1 - Calbiochem;4-Amino-5-methylamino-2′,7′-difluorescein;4-AMINO-5-METHYLAMINO-2',7'-DIFLUOROFLUORESCEIN;3-AMINO, 4-AMINOMETHYL-2',7'-DIFLUOROFLUORESCEIN;3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein
- CBNumber
- CB6679611
- Molecular Formula
- C21H14F2N2O5
- Formula Weight
- 412.34
- MOL File
- Mol file
DAF-FM Property
- Melting point:
- 265 ℃
- storage temp.
- -20°C
- solubility
- DMSO: >1mg/mL
- form
- Solid
- pka
- 8.19 ± 0.20, most acidic, temperature: 25 °C; 4.23 ± 0.20, most basic(at 25℃)
- color
- clear colorless to yellow
- Appearance
- yellow to brown solid
- λmax
- 487 nm, 495 nm
- Biological Applications
- Nitric oxide indicator; detecting microorganisms; measuring Ga i-coupled or Gao-coupled receptors activity
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H302Harmful if swallowed
- Precautionary statements
-
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P270Do not eat, drink or smoke when using this product.
P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.
P330Rinse mouth.
P501Dispose of contents/container to..…
N-Bromosuccinimide Price
- Product number
- D1821
- Product name
- DAF-FM
- Purity
- ≥98% (HPLC)
- Packaging
- 1mg
- Price
- $579
- Updated
- 2025/07/31
- Product number
- 251515-M
- Product name
- DAF-FM - CAS 254109-20-1 - Calbiochem
- Purity
- A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.
- Packaging
- 251515-1MG
- Price
- $999
- Updated
- 2024/03/01
- Product number
- D2196
- Product name
- DAF-FM solution
- Purity
- 5 mM in DMSO, ≥97% (HPLC)
- Packaging
- 1mg
- Price
- $758
- Updated
- 2025/07/31
- Product number
- CDX-A0523-M001
- Product name
- DAF-FM solution
- Purity
- ≥98%(HPLC)
- Packaging
- 1mg
- Price
- $529
- Updated
- 2021/12/16
DAF-FM Chemical Properties,Usage,Production
Description
DAF-FM (4-amino-5-methylamino-2′,7′-difluorofluorescein) is a cell-impermeant, fluorescent probe for detecting and quantifying low concentrations of nitric oxide (NO). DAF-FM does not need to be activated by cytosolic enzymes and is suitable to detect NO in extracellular matrix.
The fluorescence quantum yield of DAF-FM is ~0.005, but it increases about 160-fold to ~0.81 after reacting with NO and forming a fluorescent benzotriazole (excitation/emission maxima at 495/515 nm) .
The NO detection limit of DAF-FM (~3 nM) is more sensitive than that of DAF-2 (~5 nM). The fluorescence of the NO adduct of DAF-FM is independent of pH above pH 5.5. Moreover, the NO adduct of DAF-FM demonstrates a significantly enhanced photostability compared to that of DAF-2, ensuring reliable results and additional time for imaging.
DAF-FM should be dissolved in DMSO and then used to prepare a working solution. Buffers containing bovine serum albumin (BSA) or phenol red can affect the fluorescence and should be used cautiously.
The cell-permeant version of DAF FM — DAF-FM DA is also available.
Uses
DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.
Uses
DAF-FM is important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole.
Uses
DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells.
Biochem/physiol Actions
DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.
DAF-FM Preparation Products And Raw materials
Raw materials
Preparation Products
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