DAF-FM

Product Name: DAF-FM
CAS No.: 254109-20-1
Chemical Name: DAF-FM
Synonyms: DAF-FM;DAF-FM solution;DAF-FM >=98% (HPLC);DIAMINOFLUORESCEIN-FM;PPRXFLLOWKZHMK-UHFFFAOYSA-N;DAF-FM - CAS 254109-20-1 - Calbiochem;4-Amino-5-methylamino-2′,7′-difluorescein;4-AMINO-5-METHYLAMINO-2',7'-DIFLUOROFLUORESCEIN;3-AMINO, 4-AMINOMETHYL-2',7'-DIFLUOROFLUORESCEIN;3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein
CBNumber: CB6679611
Molecular Formula: C21H14F2N2O5
Formula Weight: 412.34
MOL File: Mol file

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DAF-FM Property

Melting point:: 265 ℃
storage temp.: -20°C
solubility: DMSO: >1mg/mL
form: Solid
pka: 8.19 ± 0.20, most acidic, temperature: 25 °C; 4.23 ± 0.20, most basic(at 25℃)
color: clear colorless to yellow
λmax: 487 nm, 495 nm
Biological Applications: Nitric oxide indicator; detecting microorganisms; measuring Ga i-coupled or Gao-coupled receptors activity

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Safety

Hazard Codes: Xn
Risk Statements: 22
WGK Germany: 3

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

P330Rinse mouth.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: D1821
Product name: DAF-FM
Purity: ≥98% (HPLC)
Packaging: 1mg
Price: $590
Updated: 2024/03/01

Sigma-Aldrich

Product number: 251515-M
Product name: DAF-FM - CAS 254109-20-1 - Calbiochem
Purity: A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.
Packaging: 251515-1MG
Price: $999
Updated: 2024/03/01

Sigma-Aldrich

Product number: D2196
Product name: DAF-FM solution
Purity: 5 mM in DMSO, ≥97% (HPLC)
Packaging: 1mg
Price: $737
Updated: 2024/03/01

Adipogen Life Sciences

Product number: CDX-A0523-M001
Product name: DAF-FM solution
Purity: ≥98%(HPLC)
Packaging: 1mg
Price: $529
Updated: 2021/12/16

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DAF-FM Chemical Properties,Usage,Production

Uses

DAF-FM is important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole.

Uses

DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells.

Uses

DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.

Biochem/physiol Actions

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.

DAF-FM Preparation Products And Raw materials

Raw materials

Preparation Products

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DAF-FM Suppliers

China 26 Switzerland 2 United Kingdom 1 United States 5 Global 34

Sigma-Aldrich

Tel: 021-61415566 800-8193336
Email: orderCN@merckgroup.com
Country: China
ProdList: 51456
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Alfa Chemistry

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Fax: 1-516-927-0118
Email: support@alfa-chemistry.com
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Shanghai EFE Biological Technology Co., Ltd.

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Alfa Chemistry

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Chongqing Chemdad Co., Ltd

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Email: sales@chemdad.com
Country: China
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Energy Chemical

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Krain Pharmaceutical Technology CO.,Ltd

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Henan Alpha Chemical Co., Ltd.

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254109-20-1, DAF-FMRelated Search:

DAF-FM

DIAMINOFLUORESCEIN-FM

4-AMINO-5-METHYLAMINO-2',7'-DIFLUOROFLUORESCEIN

3-AMINO, 4-AMINOMETHYL-2',7'-DIFLUOROFLUORESCEIN

4-Amino-5-methylamino-2′,7′-difluorescein

DAF-FM solution

4-amino-5-methylamino-20,70-difluorodifluorofluorescein

PPRXFLLOWKZHMK-UHFFFAOYSA-N

DAF-FM - CAS 254109-20-1 - Calbiochem

3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein

Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 4-amino-2',7'-difluoro-3',6'-dihydroxy-5-(methylamino)-

DAF-FM >=98% (HPLC)

4-amino-2',7'-difluoro-3',6'-dihydroxy-5-(methylamino)-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one

254109-20-1

C21H14F2N2O5

Xanthene