ChemicalBook > CAS DataBase List > METHYLENE BLUE

METHYLENE BLUE

Product Name: METHYLENE BLUE
CAS No.: 122965-43-9
Chemical Name: METHYLENE BLUE
Synonyms: METHYLENE BLUE HYDRATE;CI 52015;CINO 52015;Neisser's stain A;C.I. 52015 hydrate;MethyleneblueHydrat;MethyleneBluexhydrate;Basic Blue 9~C.I. 52015;METHYLENE BLUE USP/EP/BP;METHYLENE BLUE EXTRA PURE
CBNumber: CB6748861
Molecular Formula: C16H20ClN3OS
Formula Weight: 337.87
MOL File: 122965-43-9.mol

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METHYLENE BLUE Property

Melting point:: 190 °C
storage temp.: 2-8°C
solubility: H2O: soluble4mg/4ml
form: Fine Crystalline Powder
Colour Index: 52015
color: Green
Water Solubility: Soluble in water, alcohol, acetic acid, glycerol and chloroform. Insoluble in ether.
λmax: 661 nm
Merck: 14,6060
BRN: 3599847
InChI: InChI=1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
InChIKey: WQVSELLRAGBDLX-UHFFFAOYSA-M
SMILES: N(C1C=CC2=NC3=CC=C(N(C)C)C=C3[S+]=C2C=1)(C)C.[Cl-].O

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Safety

Hazard Codes: Xn
Risk Statements: 22
Safety Statements: 26-39-61
RIDADR: UN 2811 6.1/PG 3
WGK Germany: 3
RTECS: SO5600000
TSCA: Yes
HS Code: 32041300

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: PHR3838
Product name: Methylene Blue
Purity: certified reference material, pharmaceutical secondary standard
Packaging: 1g
Price: $188
Updated: 2025/07/31

Sigma-Aldrich

Product number: M1800900
Product name: Methylthioninium chloride
Purity: European Pharmacopoeia (EP) Reference Standard
Packaging: 130 mg
Price: $156
Updated: 2025/07/31

Sigma-Aldrich

Product number: 28514
Product name: Methylene Blue hydrate
Purity: ≥95% (calc. to the dried substance)
Packaging: 100g
Price: $96
Updated: 2025/07/31

Sigma-Aldrich

Product number: 28514
Product name: Methylene Blue hydrate
Purity: ≥95% (calc. to the dried substance)
Packaging: 1kg
Price: $640
Updated: 2025/07/31

Sigma-Aldrich

Product number: 1428008
Product name: Methylene blue
Purity: United States Pharmacopeia (USP) Reference Standard
Packaging: 750mg
Price: $467
Updated: 2025/07/31

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METHYLENE BLUE Chemical Properties,Usage,Production

Chemical Properties

green fine crystalline powder

Uses

Methylene blue is used as a dye for a number of different staining procedures, such as Wright's stain and Jenner's stain. Since it is a temporary staining technique, methylene blue can also be used to examine RNA or DNA under the microscope or in a gel. I

Uses

Methylene blue is used as a dye in different staining procedures viz. Wright's stain and Jenner's stain. It also serves as an indicator and medicine. Further, it is used to examine RNA and DNA under the microscope or in a gel. It is widely used as a redox indicator in analytical chemistry. It acts as a photosensitizer utilized to generate singlet oxygen in order to make organic peroxides by a Diels-Alder reaction. It is also used in the sulfide analysis. It plays an important role in aquaculture to treat fungal infections.

General Description

Methylene Blue (MB) is a basic histological dye.

Biochem/physiol Actions

Methylene blue is used as a bacteriologic stain or dye and indicator. It mediates the inactivation of enveloped viruses in plasma, when exposed to visible light. It is an inhibitor of nitric oxide synthase and guanylate cyclase. It also plays a key role in medicine as an antimalarial agent, reducing agent in methemoglobinemia and inducer of apoptosis in cancer cells.

in vivo

Methylene blue (Basic Blue 9) hydrate (50 and 100 mg/kg; i.p.; once, for 25 min; male NMRI mice) reduces absent prepulse inhibition^[1].
Methylene blue hydrate (20 and 40 mg/kg; p.o.; daily, for 6 months; CaMKIIα-tTA transactivator mice) preserves cognition in mice expressing full-length pro-aggregant human Tau^[2].
Methylene blue hydrate (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces TBI-induced edema and neuroinflammation and reduces acute depression-like behavior^[3].
Methylene blue hydrate (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces the percentage of inflammatory factor^[3].

Animal Model:	Male NMRI mice^[1]
Dosage:	50 and 100 mg/kg
Administration:	Intraperitoneal injection; once, for 25 minutes
Result:	Reduced the prepulse inhibition and reduced the increase in locomotor activity caused by phencyclidine (PCP).

Animal Model:	CaMKIIα-tTA transactivator mice^[2]
Dosage:	20 and 40 mg/kg
Administration:	Oral administration; daily, for 6 months
Result:	Inhibited Tau aggregation in CaMKIIα-tTA transactivator mice.

Animal Model:	TBI-treated male BALB/c mice^[3]
Dosage:	2 mg/kg
Administration:	Intravenous injection; once, for 1 day
Result:	Decreased the level of CD14, IL-1β, TNF-α, and CCL2 mRNA.

Animal Model:	TBI-treated male BALB/c mice^[3]
Dosage:	2 mg/kg
Administration:	Intravenous injection; once, for 1 day
Result:	Reduced the percentage of myeloid (CD11b+/GR1+) cells, reduced IL-1β and enhanced IL-10 expression in microglia.

METHYLENE BLUE Preparation Products And Raw materials

Raw materials

Preparation Products

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METHYLENE BLUE Suppliers

Americas 1 Belgium 1 Brazil 1 Canada 1 China 134 Czech Republic 1 Europe 1 Germany 2 India 10 Italy 1 Japan 1 Switzerland 2 United Kingdom 1 United States 12 Global 169

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View Lastest Price from METHYLENE BLUE manufacturers

Shaanxi Dideu New Materials Co. Ltd

Product: methylene blue 122965-43-9
Price: US $0.00-0.00/kg
Min. Order: 1kg
Purity: 98%
Supply Ability: 1000kg
Release date: 2025-11-21

Career Henan Chemical Co

Product: METHYLENE BLUE 122965-43-9
Price: US $1.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 100KG
Release date: 2020-01-08

122965-43-9, METHYLENE BLUERelated Search:

CI 52015 CI-52015 CI 52015 CI 52015 CI 52015 METHYLENE BLUE CI 52015 AZURE II EOSINATE CI 52015

CI 52015

CINO 52015

METHYLENE BLUE HYDRATE

METHYLENE BLUE FOR MICROSCOPY

METHYLENE BLUE REDOX INDICATOR, REAG. PH . EUR.

METHYLENE BLUE TRIHYDRATE MOLECULAR*BIOL OGY REAGENT

METHYLENE BLUE EXTRA PURE

MethyleneblueHydrat

Methylene Blue, high purity

Methylene Blue hydrate, Methylthionini chloridum

METHYLENE BLUE HYDRATE, HIGH PURITY BIOLOGICAL STAIN, 96+%

METHYLENE BLUE HYDRATE, PURE

Methylene Blue hydrate, high purity biological stain

Methylene Blue hydrate,96+%,high purity biological stain

Basic Blue 9~C.I. 52015

C.I. 52015 hydrate

Methylene Blue hydrate[redox indicator, Reag. Ph. Eur., >=96% (calc. to the dried substance), 1038(S. No.), 52015(C.I.No)]

Methylene Blue, biological stain

3,7-Bis(dimethylamino)phenothiazin-5-ium chloride hydrate(1:1:x)

Methylene Blue hydrate, high purity biological stain, 96+% 100GR

METHYLENE BLUE USP/EP/BP

Methylthioninium chloride (M1800900)

Methylene blue, hydrate, Certified grade

Neisser's stain A

Phenothiazin-5-ium,3,7-bis(dimethylamino)-, chloride, hydrate (1:1: )

METHYLENE BLUE HYDRATE AR

METHYLENE BLUE HYDRATE For Microscopy

Methylthioninium Chloride Hydrate

3,7-Bis(dimethylamino)phenothiazin-5-ium chloride xhydrate

3,7-Bis(dimethylamino)phenothiazin-5-ium chloride xhydrat

METHYLENE BLUE ALKALINE-LOFFERS SOLUTION

Methylene Blue Impurity 18

MethyleneBluexhydrate

122965-43-9

C16H18N3SClH2O

Analytical Reagents

Microscopy Reagents

Stains and Dyes

Laser DyesStains and Dyes

Organic Electronics and Photonics

Photonic and Optical Materials

Stains&Dyes, A to

Nitric Oxide and Cell Stress

Nitric Oxide Metabolism

Nitric Oxide Scavengers

Nitric Oxide ScavengersCell Signaling Enzymes

Indicators

Redox Indicators

Titration