ECOTHIOPATE IODIDE Property

Melting point:

138° (Tammelin); mp 124-124.5° (Fitch)

storage temp. 

Store at -20°C

solubility 

DMSO: Slightly Soluble; Methanol: Slightly Soluble; Water: Slightly Soluble

form 

A solid

Stability:

Hygroscopic

EPA Substance Registry System

Ethanaminium, 2-[(diethoxyphosphinyl)thio]-N,N,N-trimethyl-, iodide (513-10-0)

Safety

RIDADR 

3249

HazardClass 

6.1(a)

PackingGroup 

II

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H300Fatal if swallowed

H400Very toxic to aquatic life

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P271Use only outdoors or in a well-ventilated area.

P273Avoid release to the environment.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P301+P310IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P311Call a POISON CENTER or doctor/physician.

P312Call a POISON CENTER or doctor/physician if you feel unwell.

P321Specific treatment (see … on this label).

P330Rinse mouth.

P391Collect spillage. Hazardous to the aquatic environment

P403+P233Store in a well-ventilated place. Keep container tightly closed.

P405Store locked up.

P501Dispose of contents/container to..…

ECOTHIOPATE IODIDE Chemical Properties,Usage,Production

Originator

Phospholine Iodide,Ayerst,US,1959

Uses

Echothiophate is a phosphorylthiocholine with pharmacological action analogous to that of isofluorophate; however, spontaneous reduction of phosphorylated enzyme occurs faster after using isofluorophate. It is used in various forms of glaucoma.

Uses

Cholinergic (ophthalmic).

Definition

ChEBI: The iodide salt of ecothiopate. An irreversible acetylcholinesterase inhibitor, it is used an ocular antihypertensive in the treatment of open-angle glaucoma, particularly when other drugs have proved inadequate.

Manufacturing Process

The reaction is carried out in an atmosphere of nitrogen. To a solution of 4.60 grams sodium (0.20 mol) in 60 cc of methanol is added 14.17 grams β- dimethylaminoethyl mercaptan hydro chloride (0.10 mol), rinsed in with 10 cc methanol. Solvent is removed at a water-pump vacuum while blowing with a slow stream of nitrogen to 100°C/20 mm. To the residue suspended in 150 cc benzene and cooled in an ice bath is added 17.25 grams diethylchlorophosphate (0.10 mol) in 3 portions at 10-minute intervals. After each addition, the temperature increases from about 4° to about 14°C and then falls. The mixture is stirred in an ice bath for one-half hour and while warming to room temperature during 2 hours is washed with 35 and 5 cc portions of water with two 10 cc portions of saturated brine and is dried over calcium sulfate and filtered.
After removal of solvent by distillation under reduced pressure to 55°C/20 mm, the residue is 23.0 grams crude base (95% theory) as a pale yellow liquid. A sample of the crude base distills with some decomposition at 105° to 112°C/0.8 mm.
A sample of distilled base in cold isopropanol is treated with excess methyl iodide, left at room temperature overnight, diluted with 5 volumes of ethyl acetate and filtered from the methiodide salt. This is purified by crystallization from mixtures of isopropanol and ethyl acetate, filtering hot to remove an impurity of low solubility. The pure methiodide is obtained as a white solid, MP 124° to 124.5°C, containing 99 mol percent thiol isomer.

brand name

Phospholine Iodide (Wyeth).

Therapeutic Function

Cholinergic (ophthalmic)

Clinical Use

Echothiophate iodide has found therapeutic application for the treatment of glaucoma and strabismus. Echothiophate is applied topically as a solution and is the only irreversible AChEI for the treatment of glaucoma. The decrease in intraocular pressure observed can last up to 4 weeks. Phosphoester AChEIs exhibit cataractogenic properties; thus, their use should be reserved for patients who are refractory to other forms of treatment (i.e., short-acting miotics, β-blockers, epinephrine, and possibly, carbonic anhydrase inhibitors). Because of its toxicity, echothiophate is not used for its systemic action. Selectivity of echothiophate for the AChE catalytic site was enhanced by incorporation in the molecule of a quaternary ammonium salt functional group two carbons removed from the phosphoryl group.

Synthesis

Echothiophate, S-(2-trimethylaminoethyl)-O,O-diethylthiophosphate (13.2.23), is made by reacting diethylchlorophosphoric acid with 2-dimethylaminoethylmercaptane, giving S-(2-dimethylaminoethyl)-O,O-diethylthiophosphate (13.2.22), which is alkylated by methyliodide, forming echothiophate (13.2.23) [51].