METHYL GREEN Property

Melting point:

>300°C

solubility 

Solubility Soluble in water, ethanol; insoluble in xylene

form 

powder

color 

Green crystalline

PH Range

Yellow (0.1) to greenish-blue (2.3)

λmax

635nm, 420nm

Major Application

Liquid crystal display device, photography, recording materials, inks, cosmetics, DNA staining, cancer detection

Hazard and Precautionary Statements (GHS)

METHYL GREEN Chemical Properties,Usage,Production

Definition

ChEBI: Methyl green is an iminium salt composed of (4-{[4-(dimethylamino)phenyl][4-(trimethylazaniumyl)phenyl]methylidene}cyclohexa-2,5-dien-1-ylidene)(dimethyl)ammonium and chloride ions in a 1:2 ratio. A histological dye used to demonstrate nucleic acids by the Unna-Pappenheim stain, in conjunction with Pyronin Y. It has a role as a fluorochrome and a histological dye. It is an organic chloride salt, an iminium salt and a quaternary ammonium salt. It contains a methyl green(2+).

Synthesis

Cupric oxide is heated with 8% acetic acid, As2O3 is added, and the mixture is refluxed for two hours. The product is allowed to cool for half an hour, filtered, washed and dried.Alternate method: Dilute acetic acid is allowed to react with an excess of freshly precipitated Cu(OH)2 and the product is separated by filtration. Dilute acetic acid is added to a solution of As2O3 in boiling NaOH until the color of phenolphthalein disappears. The hot solutions are mixed (mole ratio of CuO:As2O3 = 4:3) and allowed to stand for several days [S. Avery, J. Amer. Chem. Soc. 28, 1159 (1906)].