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iodoHoechst 33258

Product Name
iodoHoechst 33258
CAS No.
158013-41-3
Chemical Name
iodoHoechst 33258
Synonyms
IODOHOECHST 33258, BR;ortho-iodoHoechst 33258;ortho-iodoHoechst 33258, BR;2,6'-Bi-1H-benzimidazole, 2'-(2-iodophenyl)-6-(4-methyl-1-piperazinyl)-;2'-(2-Iodophenyl)-6-(4-methylpiperazin-1-yl)-1H,3'H-2,5'-bibenzo[d]imidazole;2-(2-iodophenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole
CBNumber
CB82666671
Molecular Formula
C25H23IN6
Formula Weight
534.39
MOL File
158013-41-3.mol
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iodoHoechst 33258 Property

Boiling point:
744.4±70.0 °C(Predicted)
Density 
1.587±0.06 g/cm3(Predicted)
solubility 
25℃: DMSO or water Protect from light
form 
Powder
pka
10.58±0.10(Predicted)
color 
Pink to red
Appearance
Solid Powder
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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iodoHoechst 33258 Chemical Properties,Usage,Production

Description

Ortho-iodoHoechst 33258, an iodinated DNA-binding bibenzimidazole, is a cell dye for DNA quantitation which sensitizes DNA and cells to UVA.

Uses

ortho-iodoHoechst 33258 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1].

References

[1] a b c "Hoechst Stains". Invitrogren (Molecular Probes).
[2] Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505. DOI:10.1177/23.7.1095650
[3] Portugal J, Waring MJ. Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68. DOI:10.1016/0167-4781(88)90079-6

iodoHoechst 33258 Preparation Products And Raw materials

Raw materials

Preparation Products

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iodoHoechst 33258 Suppliers

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158013-41-3, iodoHoechst 33258Related Search:


  • IODOHOECHST 33258, BR
  • ortho-iodoHoechst 33258, BR
  • ortho-iodoHoechst 33258
  • 2,6'-Bi-1H-benzimidazole, 2'-(2-iodophenyl)-6-(4-methyl-1-piperazinyl)-
  • 2-(2-iodophenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole
  • 2'-(2-Iodophenyl)-6-(4-methylpiperazin-1-yl)-1H,3'H-2,5'-bibenzo[d]imidazole
  • 158013-41-3