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EOSIN B

Product Name
EOSIN B
CAS No.
548-24-3
Chemical Name
EOSIN B
Synonyms
BEosi;EOSIN B;SAFROSIN;CI 45400;EOSIN BN;Eosin bl;Eosine BA;Eosine BN;EOSIN RED;Nophalin G
CBNumber
CB8309228
Molecular Formula
C20H9Br2N2NaO9
Formula Weight
604.09
MOL File
548-24-3.mol
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EOSIN B Property

Melting point:
275 °C (dec.)(lit.)
bulk density
680kg/m3
storage temp. 
Store at +5°C to +30°C.
solubility 
spirit: soluble
Colour Index 
45400
form 
Solid
color 
Very dark purple-red to dark brown
PH
6.2 (10g/l, H2O, 25℃)
Water Solubility 
Soluble in water (390 g/L at 20°C), and ethanol.
λmax
514 nm, 395 nm
Merck 
14,3602
BRN 
4121964
Stability:
Stable. Incompatible with strong oxidizing agents.
Biological Applications
Antimalarial agent; protein assay; detecting enzyme activity; treating cancer,malaria,diabetes,a variety of conditions affecting skin,mouth,digestive tract,urinary tract,reproductive tract,respiratory tract,circulatory sys
Major Application
diagnostic assay manufacturing
hematology
histology
InChI
1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
InChIKey
GYYTYUGGVBYJHE-UHFFFAOYSA-L
SMILES
[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O
EPA Substance Registry System
Eosin B (548-24-3)
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Safety

Hazard Codes 
Xn
Risk Statements 
22
Safety Statements 
22-24/25
WGK Germany 
3
TSCA 
TSCA listed
HS Code 
3204 12 00
Storage Class
11 - Combustible Solids
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H318Causes serious eye damage

Precautionary statements

P280Wear protective gloves/protective clothing/eye protection/face protection.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P310Immediately call a POISON CENTER or doctor/physician.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
45260
Product name
Eosin B
Purity
formicroscopy(Fl.,Hist.),adsorptionandfluorescentindicator
Packaging
100g
Price
$432
Updated
2024/03/01
Sigma-Aldrich
Product number
45260
Product name
Eosin B
Purity
formicroscopy(Fl.,Hist.),adsorptionandfluorescentindicator
Packaging
25g
Price
$153
Updated
2023/06/20
Sigma-Aldrich
Product number
45260
Product name
Eosin B
Purity
for microscopy (Fl., Hist.), adsorption and fluorescent indicator
Packaging
25g-f
Price
$109
Updated
2021/12/16
Sigma-Aldrich
Product number
45260
Product name
Eosin B
Purity
for microscopy (Fl., Hist.), adsorption and fluorescent indicator
Packaging
100g-f
Price
$336
Updated
2021/12/16
TCI Chemical
Product number
E0204
Product name
Acid Red 91
Packaging
25g
Price
$32
Updated
2025/07/31
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EOSIN B Chemical Properties,Usage,Production

Chemical Properties

odourless green to reddish-brown solid

Uses

Eosin B sodium salt is a cell and tissue stain. Eosin B sodium salt is used as a counter stain for collagen, as a tissue stain for cell granules, nuclei, and microorganisms when coupled with Azure A (sc-203729), and as a stain for cell differentiation of the anterior pituitary.

Uses

Dyeing wool, cotton, and paper. In histology as a stain for epithelia, muscular fibers, nuclei, etc.

Definition

ChEBI: An organic sodium salt which is the disodium salt of eosin b diphenol.

Preparation

will 4′,5′-Dibromofluorescein(C.I. Acid Orange 11, C.I. 45370) nitrification, and translated into sodium salt or ammonium salt.

Biological Activity

Eosin B is a xanthene dye and is also referred to as eosin bluish or erythrosin B. It is used in the Harris stain for Negri bodies. Eosin B is also used in Mayer′s Hemalum as a counterstain for collagen. Eosin B-Azure A are used together for the staining of cell granules, nuclei and microorganisms. It can also be used for protein estimation. The protein - eosin B complex absorbs at wavelength range of 536–544nm and the change in blue shift helps in determining the protein concentration.

storage

Room temperature

Purification Methods

Free it from inorganic halides by repeated crystallisation from butan-1-ol. [Beilstein 19/6 V 469.]

Properties and Applications

colourful red blue light.

Standard Light Fastness Soaping Persperation Fastness Oxygen bleaching Fastness to seawater
Fading Stain Fading Stain Fading Stain
ISO 3 3 3 3 3

EOSIN B Preparation Products And Raw materials

Raw materials

Preparation Products

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EOSIN B Suppliers

Americas 1 Belgium 1 Canada 3 China 174 Europe 4 France 1 Germany 5 India 13 Japan 3 Switzerland 1 United Kingdom 4 United States 23 Global 233
Guangzhou City Haizhu District Zhenghong Co.Ltd.
Tel
020-82550624 13016020539
Fax
020-82571650
Email
2336504938@qq.com
Country
China
ProdList
419
Advantage
58
J & K SCIENTIFIC LTD.
Tel
18210857532; 18210857532
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
Advantage
76
Alfa Aesar
Tel
400-6106006
Fax
021-67582001/03/05
Email
saleschina@alfa-asia.com
Country
China
ProdList
30123
Advantage
84
TCI (Shanghai) Development Co., Ltd.
Tel
021-67121386
Fax
021-67121385
Email
Sales-CN@TCIchemicals.com
Country
China
ProdList
24529
Advantage
81
Energy Chemical
Tel
021-021-58432009 400-005-6266
Fax
021-58436166
Email
sales8178@energy-chemical.com
Country
China
ProdList
44802
Advantage
61
Shandong Xiya Chemical Co., Ltd
Tel
13355009207 13355009207
Fax
0539-6365991
Email
3007715519@qq.com
Country
China
ProdList
18729
Advantage
57
Sichuan Kulinan Technology Co., Ltd
Tel
400-1166-196 18981987031
Fax
028-84555506 800101999
Email
cdhxsj@163.com
Country
China
ProdList
11705
Advantage
57
Tianjin heowns Biochemical Technology Co., Ltd.
Tel
400 638 7771
Email
sales@heowns.com
Country
China
ProdList
14435
Advantage
57
Sinopharm Chemical Reagent Co,Ltd.
Tel
86-21-63210123
Fax
86-21-63290778 86-21-63218885
Email
sj_scrc@sinopharm.com
Country
China
ProdList
9815
Advantage
79
Maya High Purity Chemicals
Tel
+86 (573) 82222445 (0)18006601000 452520369
Fax
+86 (573) 82222643
Email
sales@maya-r.com
Country
China
ProdList
11707
Advantage
57
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View Lastest Price from EOSIN B manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
Eosin B 548-24-3
Price
US $0.00/KG
Min. Order
1KG
Purity
98%min
Supply Ability
30tons/month
Release date
2023-02-08
R&D Scientific Inc.
Product
Acid Red 548-24-3
Price
US $350.00/g
Min. Order
1g
Purity
98
Supply Ability
500 Kg
Release date
2024-02-18
Career Henan Chemical Co
Product
Eosine I Bluish 548-24-3
Price
US $1.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
25kg
Release date
2019-07-09

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