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L-(+)-Ergothioneine

Product Name
L-(+)-Ergothioneine
CAS No.
497-30-3
Chemical Name
L-(+)-Ergothioneine
Synonyms
ERGOTHIONEINE;ERGOLD;THIONEINE;Ergothionine;2-MERCAPTOHISTIDINE BETAINE;(S)-[1-carboxy-2-(2-mercaptoimidazol-4-yl)ethyl]trimethylammonium hydroxide;(S)-ALPHA-CARBOXY-2,3-DIHYDRO-N,N,N-TRIMETHYL-2-THIOXO-1H-IMIDAZOLE-4-ETHANAMINIUM INNER SALT;Ergotin;NSC 7175;Thiasine
CBNumber
CB8460398
Molecular Formula
C9H15N3O2S
Formula Weight
229.3
MOL File
497-30-3.mol
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L-(+)-Ergothioneine Property

Melting point:
275-277°C (dec.)
Density 
1.2541 (rough estimate)
refractive index 
1.6740 (estimate)
storage temp. 
-20°C
solubility 
Soluble in Water (up to 10 mg/ml)
form 
White solid.
color 
White or off-white
PH
+47^o (c=1 in water)
Stability:
Stable for 1 year from date of purchase as supplied. Solutions in water may be stored at -20°C for no more then 1 day.
InChI
InChI=1/C9H15N3O2S/c1-12(2,3)7(8(13)14)4-6-5-10-9(15)11-6/h5,7H,4H2,1-3H3,(H2-,10,11,13,14,15)/t7-/s3
InChIKey
SSISHJJTAXXQAX-KPOCXSGKNA-N
SMILES
[N+](C)(C)(C)[C@@H](CC1=CNC(S)=N1)C([O-])=O |&1:4,r|
EPA Substance Registry System
1H-Imidazole-4-ethanaminium, .alpha.-carboxy-2,3-dihydro-N,N,N-trimethyl-2-thioxo-, inner salt, (.alpha.S)- (497-30-3)
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Safety

WGK Germany 
3
HS Code 
2933299090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
E7521
Product name
L-(+)-Ergothioneine
Packaging
5mg
Price
$271
Updated
2024/03/01
Sigma-Aldrich
Product number
E7521
Product name
L-(+)-Ergothioneine
Packaging
25mg
Price
$1160
Updated
2024/03/01
Alfa Aesar
Product number
J67861
Product name
L-(+)-Ergothioneine
Purity
98+%
Packaging
250mg
Price
$123
Updated
2023/06/20
Alfa Aesar
Product number
J67861
Product name
L-(+)-Ergothioneine
Purity
98+%
Packaging
1g
Price
$391
Updated
2023/06/20
Cayman Chemical
Product number
14905
Product name
L-(+)-Ergothioneine
Purity
≥98%
Packaging
5mg
Price
$62
Updated
2024/03/01
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L-(+)-Ergothioneine Chemical Properties,Usage,Production

Description

L-(+)-Ergothioneine is a naturally-occurring amino acid derived from histidine via hercynine. Ergothioneine is a stable antioxidant that scavenges and detoxifies free radicals and oxidants, increases intracellular thiol levels, controls nuclear factor-κB activation, and inhibits inflammatory gene expression. In addition, it inhibits the peroxynitrite-dependent nitration of nitrotyrosine, blocks oxidative DNA damage and cell death, and prevents the formation of xanthine and hypoxanthine. Ergothioneine is transported by the organic cation/carnitine transporter 1, which has been linked with autoimmune diseases, including rheumatoid arthritis and Crohn’s disease.

Chemical Properties

White Solid

Uses

L-(+)-Ergothioneine is a natural molecule isolated from the rye ergot fungus and later identified in rat erythrocytes and liver and in numerous other animal tissues. Its antioxidizing properties may afford the compound therapeutic potential or it may be used as a food additive or in cosmetics.

Definition

ChEBI: Ergothioneine is a L-histidine derivative that is N(alpha),N(alpha),N(alpha)-trimethyl-L-histidine in which the hydrogen at position 2 on the imdazole ring is replaced by a mercapto group. A naturally occurring metabolite of histidine synthesized by bacteria and fungi with antioxidant properties. It is found ubiquitously in plants and animals and is present in many human foodstuffs. It has a role as an antioxidant, a fungal metabolite, a plant metabolite, a xenobiotic metabolite and a chelator. It is an amino-acid betaine, a L-histidine derivative and a sulfur-containing amino acid. It is a conjugate base of an ergothioneine(1+). It is a tautomer of an ergothioneine thione form.

Origin

Ergothioneine was discovered in 1909 by Charles Tanret, a French pharmacist and chemist. Tanret was examining the ergot fungus, which had recently been responsible for destroying crops, and he discovered the compound by using a purification process. The amino acid name ergothioneine originates from this fungus. Though this discovery is relatively recent, scientists speculate that ergothioneine may have originated from ancient earth. Due to its anaerobic nature (it does not require oxygen to function), it may have manifested in the earth's oxygen-free atmosphere more than three billion years ago While ergothioneine is not classified as one of the nine essential amino acids.

benefits

L-(+)-Ergothioneine is a natural antioxidant, which has various physiological functions such as scavenging free radicals, detoxification, maintaining DNA biosynthesis, normal cell growth and cellular immunity.

General Description

L-(+)-Ergothioneine (ET) is a sulfur-containing amino acid, which is only produced by Actinomycetales bacteria and non-yeast like fungi belonging to the division Basidiomycota and Ascomycota. It was originally isolated from Claviceps purpurea or rye ergot. It is obtained from L-histidine, which is converted into betaine form called hercynine. It is found in both animals and plants, and mammals usually obtain it from their diet, e.g. through mushrooms or oats. It is tautomeric in nature, and in neutral aqueous solution exists in thione form.

General Description

L-(+)-Ergothioneine (ERG)  is a substance that cannot be synthesized by humans and must be obtained from food. It has cytoprotective and antioxidant properties.

Biochem/physiol Actions

L-(+)-Ergothioneine (ET) has the maximum concentrations in tissues subjected to oxidative stress, with the highest being in blood, eye lens, bone marrow, semen and liver. It acts as an anti-oxidant and prevents apoptosis, by scavenging reactive oxygen and nitrogen species. The anti-oxidant activity is attributable to sulfhydryl groups. It acts as a substrate for SLC22A4 (solute carrier family 22, member 4) transporter. In alveolar macrophages, it prevents the release of interleukin-8 (IL-8) by tumor necrosis factor (TNF)α. IL-8 is an inflammatory cytokine. It also regulates the oxidative damage in liver and kidneys, and has a protective action against lipid peroxidation. It is also responsible for the conservation of endogenous glutathione and α-tocopherol. ET being an antioxidant, protects against γ and UV radiation. In UV-irradiated human dermal fibroblasts, it scavenges reactive oxygen species (ROS), and suppresses matrix metalloproteinases 1 (MMP1) expression. It might also have anti-ageing effects on skin caused by UV-radiation.

Synthesis

L-(+)-Ergothioneine is prepared by the reaction of hercynine. The steps are as follows:
15g of compound was added to 150ml of water, and 15.6g of concentrated hydrochloric acid was added, add 10.9g dibromohydantoin, stir for 20min, add D-cysteine, Continue to stir for 1 hour, add sodium thiosulfate, raise the temperature to 90-100°C, and continue the reaction for 15 hours. After the reaction, cool down and filter, adjust the pH to neutral, desalt, concentrate, crystallize with 5ml of water and 75ml of isopropanol, and dissolve the solid Filter and dry at 70-80°C to obtain 88% ergothioneine product with a yield of 81%.

Synthesis

The synthesis of L-(+)-Ergothioneine (ET; marketed as Ergoneine®) is performed in water and includes the following steps. First, l-hercynine is reacted with bromine and then with cysteine. The intermediate obtained is transformed into ET by heating in the presence of mercaptopropionic acid. Finally, the raw product is purified by crystallisation.

References

[1] Williamson R, et al. L-(+)-Ergothioneine Significantly Improves the Clinical Characteristics of Preeclampsia in the Reduced Uterine Perfusion Pressure Rat Model. Hypertension , 2022; 75.
[2] Hoek S, et al. Engineering the Yeast Saccharomyces cerevisiae for the Production of L-(+)-Ergothioneine. Frontiers in Bioengineering and Biotechnology, 2019.

L-(+)-Ergothioneine Preparation Products And Raw materials

Raw materials

Preparation Products

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L-(+)-Ergothioneine Suppliers

Tangshan Huanhe Technology Co., Ltd
Tel
13615473007 13615473007
Email
760076777@qq.com
Country
China
ProdList
20
Advantage
58
Shenzhen Hygieia Biotechnology Co., Ltd
Tel
0755-0755-27208059 13342904611
Fax
0755-27208059
Email
Alex@hygieia.com.cn
Country
China
ProdList
51
Advantage
58
Shanghai YG Ingredients Co., Ltd.
Tel
021-61453999-2383 18918615499
Email
sales@ygingredients.com
Country
China
ProdList
2
Advantage
58
Shanghai AECOM Biotechnology Co. , Ltd.
Tel
021-021-20956531 18116226605
Fax
021-20956532
Email
sui@neosunpharm.com
Country
China
ProdList
350
Advantage
55
Shanghai CoachChem Co., Ltd.
Tel
021-67299971
Fax
021-67299972
Email
marketing@coachchem.com
Country
China
ProdList
85
Advantage
62
Wuxi Jingyao Bio-Technology Co., Ltd.
Tel
0510-88770633 13961738808
Email
jingyaobiotech@126.com
Country
China
ProdList
324
Advantage
55
InnovaSyn
Tel
025-13357828857 13357828857
Email
vivi.wu@innovagt.com
Country
China
ProdList
15
Advantage
58
Biopole Pharmatech Co., Ltd.
Tel
0512-65974739-0 15151475053
Email
mengqi_biopole@163.com
Country
China
ProdList
45
Advantage
58
Hubei Baoling Chemical Technology Co. , Ltd.
Tel
13129969326
Email
2797487194@qq.com
Country
China
ProdList
1007
Advantage
58
Shandong Yuanlitai Nutratech Co., Ltd,
Tel
18980869808
Email
info@yltnutra.com
Country
China
ProdList
9
Advantage
58
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View Lastest Price from L-(+)-Ergothioneine manufacturers

CONTIDE BIOTECH CO.,LTD
Product
L-(+)-Ergothioneine 497-30-3
Price
US $0.00/G
Min. Order
1G
Purity
99%
Supply Ability
20
Release date
2024-03-27
HEBEI SHENGSUAN CHEMICAL INDUSTRY CO.,LTD
Product
L-(+)-Ergothioneine 497-30-3
Price
US $999.00-666.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
5000
Release date
2024-08-20
HEBEI SHENGSUAN CHEMICAL INDUSTRY CO.,LTD
Product
L-(+)-Ergothioneine 497-30-3
Price
US $500.00-300.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
5000
Release date
2024-08-23

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