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BES

Product Name
BES
CAS No.
10191-18-1
Chemical Name
BES
Synonyms
N,N-BIS(2-HYDROXYETHYL)-2-AMINOETHANESULFONIC ACID;BES;abs9128;Nsc166667;BES, ≥99%(T);BES ULTRAPURE;BES, ≥99.5%(T);99% purity BES;BES, SIGMAULTRA;bes(bufferingagent)
CBNumber
CB9216172
Molecular Formula
C6H15NO5S
Formula Weight
213.25
MOL File
10191-18-1.mol
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BES Property

Melting point:
150-155 °C
Density 
1.210 (estimate)
vapor pressure 
0.001Pa at 25℃
refractive index 
1.5500 (estimate)
storage temp. 
room temp
solubility 
H2O: 1 M at 20 °C, clear, colorless
form 
Powder
color 
White
PH
2.5-5.0 (25℃, 1M in H2O)
pka
7.1(at 25℃) 7.15(at 20℃)
PH Range
6.4 - 7.8
Water Solubility 
Soluble
λmax
λ: 260 nm Amax: 0.095
λ: 280 nm Amax: 0.080
BRN 
1781572
LogP
-3.59 at 26℃
CAS DataBase Reference
10191-18-1(CAS DataBase Reference)
NIST Chemistry Reference
N,n-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid(10191-18-1)
EPA Substance Registry System
Ethanesulfonic acid, 2-[bis(2-hydroxyethyl)amino]- (10191-18-1)
Absorption
≤0.080 at 280 in H2O at 1M
≤0.095 at 260 in H2O at 1M
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26
WGK Germany 
3
RTECS 
KI7363500
TSCA 
Yes
HS Code 
29221980
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P405Store locked up.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
391334
Product name
BES, Free Acid, ULTROL® Grade
Purity
BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable.
Packaging
25G
Price
$130
Updated
2024/03/01
Sigma-Aldrich
Product number
391334
Product name
BES, Free Acid, ULTROL® Grade
Purity
BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable.
Packaging
100G
Price
$328
Updated
2024/03/01
Sigma-Aldrich
Product number
14853
Product name
BES
Purity
BioXtra, for molecular biology, ≥99.5% (T)
Packaging
50g
Price
$270
Updated
2023/06/20
Sigma-Aldrich
Product number
14853
Product name
BES
Purity
BioXtra, for molecular biology, ≥99.5% (T)
Packaging
250g
Price
$990
Updated
2023/06/20
TCI Chemical
Product number
B0909
Product name
N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic Acid [Good's buffer component for biological research]
Purity
>98.0%(T)
Packaging
25g
Price
$36
Updated
2024/03/01
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BES Chemical Properties,Usage,Production

Description

BES is a zwitterionic buffer that is used in biochemistry and molecular biology research. It is one of the "Good" buffers developed in the 1960's to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The pioneering publication by Good and co-workers describes the synthesis of BES and its physical properties.
A protocol has been reported for the use of BES buffered saline in the calcium phosphate mediated transfection of eukaryotic cells with plasmid DNA. An investigation has been published on the interaction of BES and other amine buffers with DNA and the measurement of these complexes by free solution capillary electrophoresis.
The effect of BES and other zwitterionic buffers on the bicinchoninic acid (BCA) measurement of microgram quantities of protein has been studied. BES was found to diminish color development of the proteinBCA complex by 30-35% at 50 mM, and by <10% at 5 mM, measuring in the 1-10 mg range of BSA against protein-BCA complexes formed in water. This color loss may result from binding of Cu2+ to BES.

Chemical Properties

White/clear cryst. powder

Uses

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C. Absorbance (100 mM, 260 nm):≤0.05.
BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.

Uses

BES is used as a buffer to maintain the pH of the solutions in biological experiments. It is useful for diagnostic assay manufacturing industry. It induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes. It acts as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers.

Uses

BES may be employed as binding buffer in modified Eagle′s medium during the binding assay of human melanoma cells. It may be used as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers.

Application

BES, or N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, can be used to study molecular biology, biological buffers, molecular biology reagents and zwitterionic compounds. BES has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES has also been used to determine that saline and buffers minimize the action of interfering factors in the bacterial endotoxins test.
BES is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.

Definition

ChEBI: 2-[bis(2-hydroxyethyl)amino]ethanesulfonic acid is a Good's buffer substance, pKa = 7.15 at 20 ℃. It is a BES and an amino sulfonic acid. It is a conjugate acid of a 2-[bis(2-hydroxyethyl)amino]ethanesulfonate. It is a tautomer of a 2-[bis(2-hydroxyethyl)ammonio]ethanesulfonate.

Preparation

First, sodium vinyl sulfonate and diethanolamine synthesize N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium by addition reaction. Through the strong acid ion exchange resin sodium ions, the eluent concentrated after adding methanol crystallization. Finally, BES is obtained after precipitation.

General Description

BES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.

Flammability and Explosibility

Not classified

Purification Methods

Crystallise BES from aqueous EtOH. [Beilstein 4 IV 3290.]

BES Preparation Products And Raw materials

Raw materials

Preparation Products

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BES Suppliers

Americas 1 Belgium 2 Canada 1 China 308 Europe 5 France 1 Germany 7 India 27 Japan 5 Poland 1 South Korea 1 Switzerland 3 The Netherlands 2 United Kingdom 18 United States 43 Global 425
Advent Bio, Inc.
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View Lastest Price from BES manufacturers

Hebei Mujin Biotechnology Co.,Ltd
Product
BES 10191-18-1
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
50000KG/month
Release date
2023-11-13
Watson Biotechnology Co.,Ltd
Product
BES 10191-18-1
Price
US $0.00-0.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
20MT
Release date
2024-08-22
WUHAN FORTUNA CHEMICAL CO., LTD
Product
BES 10191-18-1
Price
US $0.00-0.00/Kg/Drum
Min. Order
1KG
Purity
99%-101%
Supply Ability
1000KG
Release date
2021-06-18

10191-18-1, BESRelated Search:

2-Diethylaminoethanethiol N-ETHYLDIETHANOLAMINE HYPOTAURINE Taurine Diethanolamine 2-(DIMETHYLAMINO)ETHANETHIOL N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt N-Methyldiethanolamine BES N-METHYLTAURINE METHYLXYLENOL BLUE Dermol BES BES ,Free acid KLH, BES/Mg2+ Buffer Ethanesulfonic acid BES BUFFER BES BUFFERED SALINE BES-1 protein

  • BES ULTRAPURE
  • N,N-Bis(2-hydroxyethyl)-2-aminoethanesulphonic acid >99%
  • N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic Acid [Good's buffer component for biological research]
  • N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine
  • Bes, 99+%, for biochemistry
  • BES, for biochemistry
  • 2-[N,N-Bis(2-hydroxyethyl)amino]ethanesulfonic acid (BES)
  • Ethanesulfonic acid, 2-[bis(2-hydroxyethyl)amino]-
  • n,n-bis(2-hydroxyethyl)-taurin
  • Taurine, N,N-bis(2-hydroxyethyl)-
  • N,N-BIS(HYDROXYETHYL)-2-AMINOETHANESULFONIC ACID
  • N,N-BIS(2-HYDROXYETHYL)-2-AMINOETHANESULFONIC ACID
  • N,N-BIS(2-HYDROXYETHYL)-2-AMINOETHANESULPHONIC ACID
  • 2-[BIS(2-HYDROXYETHYL)AMINO]ETHANESULFONIC ACID
  • BES N,N-Bis(hydroxyethyl)-2-aminoethanesulfonic acid
  • BES, Free Acid, ULTROL Grade
  • BES, Molecular Biology Grade N,N-Bis(hydroxyethyl)-2-aminoethanesulfonic acid, Molecular Biology Grade
  • 2-[bis(2-hydroxyethyl)amino]ethanesulphonic acid
  • BES FREE ACID MOLECULAR BIOLOGY REAGENT
  • BES, SIGMAULTRA
  • BES, BIOTECHNOLOGY PERFORMANCE CERTIFIED, CELL CULTURE TESTED
  • BIS(2-HYDROXYETHYL)-2-AMINOETHANESULPHONIC ACID
  • BES
  • N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid(BES)
  • N,N-Bis(2-hydroxyethyl)taurine
  • Nsc166667
  • N,N-Bis(2-hydroxyeth
  • BES, for biocheMistry, 99+% 100GR
  • BES Vetec(TM) reagent grade, >=99%
  • BES buffer free acid
  • BES, BIOPERFORMANCE CERTIFIED&
  • BES, Free Acid, ULTROL
  • N, N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid, free acid
  • 2-(bis(2-hydroxyethyl)amino)-ethanesulfonicaci
  • 2-[bis(2-hydroxyethyl)amino]-ethanesulfonicaci
  • bes(bufferingagent)
  • 2-[N,N-BIS(2-HYDROXYETHYL)AMINO]ETHANESULFONIC ACID
  • BesN,N-Bis(2-Hydroxyethyl)-2-AminoethanesulfonicAcid
  • BES, for biochemistry, 99+%
  • BES,BIOLOGICALBUFFER
  • N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid
  • BES, ≥99%(T)
  • BES, ≥99.5%(T)
  • N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic Acid [Good's buffer component for biological research]&gt
  • N,N-Bis(2-hydroxyethyl)-2-aminoethanesulphonic acid, 99%, reagent grade
  • N,N-Bis(2-hydroxyethyl)-2-aminoethanesulphonic acid, 99%, for bio
  • 2-[Bis(2-hydroxyethyl)amino]ethansulfonsure
  • 99% purity BES
  • abs9128
  • N. N-di (2-hydroxyethyl) - 2-aminoethanesulfonic Acid
  • BES Buffer extrapure, 99%
  • BES Buffer for cell culture, 99%, Endotoxin (BET) 0.05EU/mg
  • 2-(diethanolamine) ethanesulfonic acid
  • BES buffer {N,N-Bis[2-hydroxyethyl]-2-aminoethanesulphonic acid}
  • BES Buffer 99% For Biochemistry
  • BES BUFFER BIOCHEMISTRY
  • 10191-18-1
  • 191-18-1