C6-NBD SPHINGOSINE, BETA-D-GLUCOSYL

C6-NBD SPHINGOSINE, BETA-D-GLUCOSYL Property

Density 

1.27±0.1 g/cm3(Predicted)

storage temp. 

−20°C

solubility 

Chloroform:Methanol (5:1): Soluble; Methanol: Soluble

form 

A solid

pka

12.92±0.70(Predicted)

color 

Yellow to orange

Appearance

Solid Powder

Safety

WGK Germany 

3

Hazard and Precautionary Statements (GHS)

C6-NBD SPHINGOSINE, BETA-D-GLUCOSYL Chemical Properties,Usage,Production

Description

C6 NBD glucosylceramide (d18:1/6:0) is a biologically active derivative of glucosylceramide that is tagged with a fluorescent C-6 nitrobenzoxadiazole (C-6 NBD; ) group. C6 NBD glucosylceramide (d18:1/6:0) has been used to study the metabolism and internalization of glucosylceramide. [Matreya, LLC. Catalog No. 1622]

Uses

C6 NBD Glucosylceramide is a glucosylceramide fluorescent derivative (Ex=466 nm, Em=535 nm). C6 NBD Glucosylceramide can be used to study the metabolism and internalization of glucosylceramide and also for the determination of glucosyl ceramide synthase activity[1][2][3].

References

[1] Kok JW, et al. Salvage of glucosylceramide by recycling after internalization along the pathway of receptor-mediated endocytosis. Proc Natl Acad Sci U S A. 1989 Dec;86(24):9896-900. DOI:10.1073/pnas.86.24.9896
[2] Hayashi Y, et al. A sensitive and reproducible assay to measure the activity of glucosylceramide synthase and lactosylceramide synthase using HPLC and fluorescent substrates. Anal Biochem. 2005 Oct 15;345(2):181-6. DOI:10.1016/j.ab.2005.05.029
[3] di Bartolomeo S, et al. Differential chemosensitizing effect of two glucosylceramide synthase inhibitors in hepatoma cells. Biochem Biophys Res Commun. 2001 Oct 19;288(1):269-74. DOI:10.1006/bbrc.2001.5748