BTC,tetrapotassiumsalt Chemical Properties

storage temp. 

Store at -20°C

solubility 

Water: Soluble

form 

A solid

BTC,tetrapotassiumsalt Usage And Synthesis

Description

BTC is a low affinity calcium indicator (K_d = 7-26 μM) that displays excitation/emission spectra of 401/529 nm, respectively. It exhibits a shift in the excitation maximum from approximately 480 to 401 nm upon calcium binding, enabling determination of ratiometric calcium measurements.¹ BTC is suitable for detecting elevated calcium levels associated with activation of smooth muscle, neurons, and intracellular calcium stores.^1,2

Uses

BTC tetrapotassium is a low affinity calcium indicator (Kd approximately 7-26 μM) featuring many desirable properties for cellular calcium imaging, including long excitation wavelengths (400/485 nm), low sensitivity to Mg2+, and accuracy of ratiometric measurement[1].

References

1. Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications ,(2010).
2. Hyrc, K.L., Bownik, J.M., and Goldberg, M.P. Neuronal free calcium measurement using BTC/AM, a low affinity calcium indicator Cell Calcium 24(3),165-175(1998).

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