STAINS-ALL
STAINS-ALL Basic information
- Product Name:
- STAINS-ALL
- Synonyms:
-
- CARBOCYANIN DBTC
- DBTC
- 3,3'-DIETHYL-9-METHYL-4,5,4',5'-DIBENZOTHIACARBOCYANINE
- 3,3'-DIETHYL-9-METHYL-4,5,4',5'-DIBENZOTHIACARBOCYANINE BROMIDE
- 3,3'-DIETHYL-9-METHYL-4,5,4',5'-DIBENZOTHIACORBOCYANINE
- 3,3'-DIETHYL-9-METHYL-4,5,4',5'-NAPHTHOTHIACARBOCYANINE BROMIDE
- 1-ETHYL-2-[3-(1-ETHYLNAPHTHO[1,2-D]THIAZOLIN-2-YLIDENE)-2-METHYLPROPENYL]NAPHTHO[1,2-D]THIAZOLIUM BROMIDE
- 1-ETHYL-2-(3-[1-ETHYLNAPHTHO(1,2-D)THIAZOLIN-2-YLIDENE]-2-METHYL-PROPENYL)NAPHTHOL(1,2-D)THIAZOLIUM BROMIDE
- CAS:
- 7423-31-6
- MF:
- C30H27BrN2S2
- MW:
- 559.58
- EINECS:
- 231-047-7
- Product Categories:
-
- Naphthothiazole
- Mol File:
- 7423-31-6.mol
STAINS-ALL Chemical Properties
- Melting point:
- >200℃
- Density
- 1.3339 (estimate)
- storage temp.
- 2-8°C
- solubility
- Soluble in water, ethanol, chloroform
- form
- Dull PurplTo Powder
- color
- Dull dark purplish to dark gray
- Water Solubility
- Soluble in water.
- Sensitive
- Light Sensitive
- λmax
- 575 nm
- BRN
- 3865213
- Biological Applications
- Detecting nucleic acid hybridization; identifying microorganisms
- CAS DataBase Reference
- 7423-31-6(CAS DataBase Reference)
- EPA Substance Registry System
- Naphtho[1,2-d]thiazolium, 1-ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazol-2(1H)-ylidene)-2-methyl-1-propenyl]-, bromide (7423-31-6)
MSDS
- Language:English Provider:SigmaAldrich
- Language:English Provider:ACROS
STAINS-ALL Usage And Synthesis
Chemical Properties
dull dark purplish to dark grey powder
Uses
Stains-all is an ideal dye for the visualization and identification of proteins on polyacrylamide gels because it differentiates between highly acidic proteins which stain blue and less acidic proteins which stain pink. When employed on polyacrylamide gels, the intensity of Stains-all can be increased by the addition of silver nitrate. It is a useful nucleic acid and protein stain.
Definition
ChEBI: Carbocyanin DBTC is an organic bromide salt and a cyanine dye. It has a role as a fluorochrome. It contains a carbocyanin DBTC(1+).
Biological Activity
Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca2+ binding proteins deep blue to violet, proteins red and lipids yellow-orange. It also stains RNA (bluish purple) and DNA (blue).
As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.
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