4-((4-(DIMETHYLAMINO)PHENYL)AZO)BENZOIC ACID, SUCCINIMIDYL ESTER Chemical Properties

Melting point:

242 °C(dec.)

Boiling point:

550.7±60.0 °C(Predicted)

Density 

1.30±0.1 g/cm3(Predicted)

storage temp. 

−20°C

solubility 

Soluble in DMSO.

form 

powder to crystal

pka

3.28±0.10(Predicted)

color 

Light yellow to Amber to Dark green

Appearance

Solid Powder

Sensitive 

Moisture Sensitive

InChI

InChI=1S/C19H18N4O4/c1-22(2)16-9-7-15(8-10-16)21-20-14-5-3-13(4-6-14)19(26)27-23-17(24)11-12-18(23)25/h3-10H,11-12H2,1-2H3

InChIKey

IBOVDNBDQHYNJI-QZQOTICOSA-N

SMILES

C(ON1C(=O)CCC1=O)(=O)C1=CC=C(N=NC2=CC=C(N(C)C)C=C2)C=C1

CAS DataBase Reference

146998-31-4

Safety Information

Safety Statements 

24/25

RIDADR 

3234

WGK Germany 

3

F 

10-21

HS Code 

29280000

MSDS

• Language:English Provider:SigmaAldrich

4-((4-(DIMETHYLAMINO)PHENYL)AZO)BENZOIC ACID, SUCCINIMIDYL ESTER Usage And Synthesis

Description

Dabcyl SE is an amine-reactive, chromophoric dye that is useful in FRET (fluorescence resonance energy transfer) applications. It is utilized in the synthesis of quenched nucleic acid probes and protease substrates. These substrates are non-fluorescent until an enzymatic event liberates the fluorophore from the quencher resulting in a fluorescent response.

Uses

Non-Fluorescent probe for labeling proteins or peptides

Uses

4-[4-(Dimethylamino)phenylazo]benzoic acid N-succinimidyl ester is a useful reagent (similar to MANCYL-SE) for labeling proteins or peptides through their amino-groups by forming stable peptide bonds. The succinimidyl ester is reactive with terminal amines or lysines of peptides and other nucleophiles for fluorescent studies of proteins. Its fluorescent properties (has a characteristic broad and intense visible absorption but has no fluorescence) make it an ideal long wavelength quencher and it has been utilized as an acceptor chromophore in FRET studies. It is also used for N-terminal modification of peptides in automated synthesis.

Definition

ChEBI: Dabcyl SE dye is a pyrrolidinone and a monoazo compound. It has a role as a fluorochrome.

reaction suitability

reagent type: fluorescent-labelling reagent

4-((4-(DIMETHYLAMINO)PHENYL)AZO)BENZOIC ACID, SUCCINIMIDYL ESTER(146998-31-4)Related Product Information

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