CypHer5 Chemical Properties

Density 

1.34±0.1 g/cm3(Predicted)

pka

-1.47±0.60(Predicted)

form 

Solid

color 

Brown to reddish brown

InChIKey

VRQVBVFJEIJSIE-UHFFFAOYSA-N

SMILES

N1(CCCCCC(O)=O)C2=C(C=C(S(O)(=O)=O)C=C2)C(C)(C)C1=CC=CC=CC1C(C)(C)C2=C(N=1)C=CC(S(O)(=O)=O)=C2

CypHer5 Usage And Synthesis

Uses

CypHer 5 is a pH-sensitive cyanine derivative and a environmental sensitive fluorophore. CypHer 5 has a pH-sensitive maximal absorption at 644 nm and emission at 664 nm. CypHer 5 has been used as a pH sensor of lysosomal compartments[1].

References

[1] Patrick MJ, et al. Intracellular pH measurements using perfluorocarbon nanoemulsions. J Am Chem Soc. 2013 Dec 11;135(49):18445-57. DOI:10.1021/ja407573m
[2] Elaine J Adie, et al. CypHer 5: a generic approach for measuring the activation and trafficking of G protein-coupled receptors in live cells. Assay Drug Dev Technol. 2003 Apr;1(2):251-9. DOI:10.1089/15406580360545062