APF Chemical Properties

Boiling point:

677.7±55.0 °C(Predicted)

Density 

1.52±0.1 g/cm3(Predicted)

storage temp. 

Store at -20°C

solubility 

DMF:20.0(Max Conc. mg/mL);47.23(Max Conc. mM)
DMSO:10.0(Max Conc. mg/mL);23.62(Max Conc. mM)
Ethanol:10.0(Max Conc. mg/mL);23.62(Max Conc. mM)
Ethanol:PBS (pH 7.2) (1:3):0.5(Max Conc. mg/mL);1.18(Max Conc. mM)

form 

Pale yellow liquid.

pka

9.61±0.20(Predicted)

color 

Colorless to light yellow

APF Usage And Synthesis

Description

APF is an aromatic amino-fluorescein derivative and fluorescent probe for highly reactive radicals. It has low intrinsic fluorescence, however, upon oxidation by hydroxyl radical, hypochlorite ion, and certain peroxidase intermediates, it is converted to the highly fluorescent molecule fluorescein, facilitating the detection of highly reactive biological radicals. APF is not oxidized by nitric oxide (NO), hydrogen peroxide (H₂O₂), or other oxidants. Fluorescein displays excitation/emission maxima of 490/515 nm, respectively.

Uses

APF is a fluorescence probe that can selectively, and dose dependently detect certain species among ROS and that are highly resistant to autoxidation. APF can be used in enzymatic and cellular systems[1].

References

[1] Setsukinai K, et al. Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species. J Biol Chem. 2003;278(5):3170-3175. DOI:10.1074/jbc.M209264200

APF(359010-70-1)Related Product Information

• ROS Probe, HPF