5'',6'':3',4']dipyrido[1,2-a:1',2'-a']diindol-5-ium, 2-[2-[(2,5-dioxo-1-pyrrolidinyl)oxy]-2-oxoethyl]-6,7,7a,8a,9,10,16,18-octahydro-16,16,18,18-tetramethyl-14-sulfo-, inner salt Chemical Properties

solubility 

good in DMF, DMSO

form 

Solid

color 

Pale purple to purple

Appearance

dark red powder

5'',6'':3',4']dipyrido[1,2-a:1',2'-a']diindol-5-ium, 2-[2-[(2,5-dioxo-1-pyrrolidinyl)oxy]-2-oxoethyl]-6,7,7a,8a,9,10,16,18-octahydro-16,16,18,18-tetramethyl-14-sulfo-, inner salt Usage And Synthesis

Description

Cyanine3B NHS ester is a reactive dye for the specific labeling of amino-groups in biomolecules — antibodies, peptides, or oligonucleotides. Cyanine3B is an improved version of yellow Cyanine3 dye with significantly increased fluorescence quantum yield and photostability.

Uses

Cy3B NHS ester is a fluorescent dye compound that is commonly used in biomarking and fluorescent labeling experiments, especially for labeling biomolecules containing amino functional groups (amine groups), such as proteins, antibodies or peptides.

Definition

ChEBI: Cy3B dye NHS ester is a fluorescent dye with a maximum emission wavelength of 572 nm, derived from a heteroheptacyclic ring system. It has a role as a fluorochrome. It is an iminium betaine, an organic heteroheptacyclic compound and a cyanine dye.

Abs/Em Maxima

559/571 nm

Fluoresene quantum yield

0.68

Extinction Coefficient

121000

CF260

0.044

CF280

0.077