FMOC-2,2,6,6-TETRAMETHYLPIPERIDINE-N-OXYL-4-AMINO-4-CARBOXYLIC ACID Chemical Properties

Melting point:

162-164°C

storage temp. 

Store at +2°C to +8°C.

solubility 

Acetonitrile (Slightly), Chloroform (Slightly), Methanol (Slightly)

form 

Solid

color 

Pale Yellow to Yellow

Safety Information

WGK Germany 

WGK 2 water endangering

HS Code 

2933 39 99

FMOC-2,2,6,6-TETRAMETHYLPIPERIDINE-N-OXYL-4-AMINO-4-CARBOXYLIC ACID Usage And Synthesis

Chemical Properties

Yellow Solid

Uses

A protected spin-labelled, cyclic, chiral ?amino acid resolved in an enantiomerically pure state

Uses

A stable free radical spin label.

Uses

Fmoc-2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxylic Acid is a stable, free radical spin label.

General Description

Fmoc-TOAC-OH is a useful tool for the incorporation of the ESR spin-label TOAC into peptide sequences [1]. Incorporation of this derivative and the following residue is best achieved using HATU activation. TFA/water/TIS should be used for cleavage of TOAC-containing peptides. The use of EDT should be avoided as it can cause permanent reduction of the nitroxide radical [2]. Following cleavage, the TOAC peptide should be treated with aqueous ammonia in air to regenerate the nitroxide from the hydroxylamine that is generated by the TFA treatment.