FMOC-2,2,6,6-TETRAMETHYLPIPERIDINE-N-OXYL-4-AMINO-4-CARBOXYLIC ACID Chemical Properties

Melting point:

162-164°C

storage temp. 

Store at +2°C to +8°C.

solubility 

Acetonitrile (Slightly), Chloroform (Slightly), Methanol (Slightly)

form 

Solid

color 

Pale Yellow to Yellow

Safety Information

WGK Germany 

WGK 2 water endangering

HS Code 

2933 39 99

FMOC-2,2,6,6-TETRAMETHYLPIPERIDINE-N-OXYL-4-AMINO-4-CARBOXYLIC ACID Usage And Synthesis

Chemical Properties

Yellow Solid

Uses

A protected spin-labelled, cyclic, chiral ?amino acid resolved in an enantiomerically pure state

Uses

A stable free radical spin label.

Uses

Fmoc-2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxylic Acid is a stable, free radical spin label.

General Description

Fmoc-TOAC-OH is a useful tool for the incorporation of the ESR spin-label TOAC into peptide sequences [1]. Incorporation of this derivative and the following residue is best achieved using HATU activation. TFA/water/TIS should be used for cleavage of TOAC-containing peptides. The use of EDT should be avoided as it can cause permanent reduction of the nitroxide radical [2]. Following cleavage, the TOAC peptide should be treated with aqueous ammonia in air to regenerate the nitroxide from the hydroxylamine that is generated by the TFA treatment.

reaction suitability

reaction type: Fmoc solid-phase peptide synthesis