MCA-PRO-LEU-GLY-LEU-DAP(DNP)-ALA-ARG-NH2 Chemical Properties

Density 

1.48±0.1 g/cm3(Predicted)

storage temp. 

−20°C

solubility 

20% acetic acid: 1mg/mL

form 

Solid

pka

12.99±0.46(Predicted)

color 

Light yellow to yellow

Appearance

Yellow Powder

InChIKey

RQASLOJQHHELIQ-QJSKOQLASA-N

Safety Information

WGK Germany 

3

MSDS

• Language:English Provider:SigmaAldrich

MCA-PRO-LEU-GLY-LEU-DAP(DNP)-ALA-ARG-NH2 Usage And Synthesis

Description

7-Methoxycoumarin-4-acetyl-P-L-G-L-β-(2,4-dinitrophenylamino)A-R amide is a fluorogenic substrate for matrix metalloproteinases (MMPs). It is ceaved by an MMP to release the fluorescent moiety 7-methoxycoumarin-4-acetyl (Mca), which can be used to quantify MMP activity.

Uses

MOCAc-PLGL(Dpa)AR is a positively charged fluorescent substrate for matrix metalloproteinase-2 (MMP-2), MMP-7 and MMP-9. MOCAc-PLGL(Dpa)AR is a substrate of matrilysin, can be cleaved at the peptide bond between the glycine and leucine residues[1][2].

Biological Activity

Cell permeable: no', 'Primary Target
Fluorogenic substrate for matrix metalloproteinases (MMPs)', 'Product does not compete with ATP.', 'Reversible: no', 'kcat/Km = 1.7 x 105 M-1sec-1 for MMP-7 and 6.3 x 105 M-¹ sec-¹ for MMP-2

References

[1] Oneda H, et al. Substrate-dependent activation of thermolysin by salt. Biosci Biotechnol Biochem. 2004 Aug;68(8):1811-3. DOI:10.1271/bbb.68.1811
[2] Oneda H, et al. Inhibitory effects of green tea catechins on the activity of human matrix metalloproteinase 7 (matrilysin). J Biochem. 2003 May;133(5):571-6. DOI:10.1093/jb/mvg073

Sequene

Mca-PLGL-Dap(Dnp)-AR-NH2

MCA-PRO-LEU-GLY-LEU-DAP(DNP)-ALA-ARG-NH2(140430-53-1)Related Product Information

• 2-Ethyl-5-nitrobenzenamine ,98%
• BIS(2-AMINOPROPYL)AMINE
• 7-Methoxycoumarin
• N,N'-Bis(2-aminoethyl)-1,3-propanediamine
• N1-(2,4-DINITRO-PHENYL)-ETHANE-1,2-DIAMINE
• 7-Methoxycoumarin
• 1-NITRO-2-PROPYLBENZENE
• 3-(2-HYDROXYPHENYL)PROPIONAMIDE
• 3-(4-NITROPHENYL)PROPAN-1-AMINE
• 3-(2-NITRO-PHENYL)-PROPIONALDEHYDE
• CHEMBRDG-BB 7932577
• MCA-PRO-LEU-GLY-LEU-DAP(DNP)-ALA-ARG-NH2
• 2-(2-NITROPHENYL)ETHANAMINE
• N-(4-NITRO-BENZYL)-FORMAMIDE
• N-(4-AMINO-BENZYL)-ACETAMIDE
• 4-(1-AMINO-ETHYL)-PHENYLAMINE
• MOCAC-PRO-LEU-GLY
• 3-[(4-AMINOPHENYL)AMINO]-PROPANAMIDE