QSY 21 CARBOXYLIC ACID, SUCCINIMIDYL ESTER Chemical Properties

Melting point:

>200℃

solubility 

Soluble in dimethyl sulfoxide, methanol

form 

Solid

color 

Purple solid

Appearance

dark purple powder

λmax

661 nm

Biological Applications

Amplification assays; hybridization assays; litigation assays; ligand assays; detecting analytes,enzymes,optical microcavities,protein– DNA interactions; imaging apoptosis,b-lactamase activity; FRET probes

QSY 21 CARBOXYLIC ACID, SUCCINIMIDYL ESTER Usage And Synthesis

Description

DusQ® 21 is a dark quencher used as an acceptor in FRET applications, it has absorption maximum 656 nm, and can be used in multiplex assays due to the lack of secondary fluorescence. It is a quencher of choice for pairing with fluorescent dyes that emit in the red and near-infrared part of the range. DusQ 21 NHS ester derivative is used for labeling with the primary amines of amine-containing molecules. It can be used for quenching of many fluorophores, including Cyanine5, Cyanine5.5, AF 647.

Uses

QSY 21 NHS, a dark quencher is an efficient energy transfer acceptor of the far red and NIR fluorescent probes. QSY 21 NHS works in the wavelength range of 540-750 nm, and is frequently used in FRET applications. QSY 21 NHS does not emit fluorescence in normal conditions. NHS esters can be used to label the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules[1].

References

[1] Kabelác M, et al. A comparative study of the binding of QSY 21 and Rhodamine 6G fluorescence probes to DNA: structure and dynamics. Phys Chem Chem Phys. 2010 Sep 7;12(33):9677-84. DOI:10.1039/c004020g

Abs/Em Maxima

656 nm

Extinction Coefficient

72800

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