N-P-TOSYL-GLY-PRO-ARG P-NITROANILIDE ACETATE SALT Chemical Properties

storage temp. 

−20°C

solubility 

DMF: 20 mg/ml; DMSO: 3 mg/ml; PBS (pH 7.2): 3 mg/ml

form 

A solid

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

F 

10-21

MSDS

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N-P-TOSYL-GLY-PRO-ARG P-NITROANILIDE ACETATE SALT Usage And Synthesis

Description

N-(p-Tosyl)-GPR-pNA is a colorimetric substrate for thrombin. Thrombin preferentially binds to and cleaves the Gly-Pro-Arg (GPR) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of thrombin activity.

Uses

N-(p-Tosyl)-GPR-pNA acetate (Chromozym-TH) is a chromogenic substrate targeting the synthetic peptides Hirunorm IV and Hirunorm V and can be used to detect the dissociation constants (KI) of both peptides. Hirunorm IV and Hirunorm V are reversible inhibitors of amidolytic thrombin activity. By varying the peptide concentration at a fixed concentration of the chromogenic substrate N-(p-Tosyl)-GPR-pNA acetate, the dissociation constants determined were 0.134 nM (Hirunorm IV) and 0.245 nM (Hirunorm V)[1].

References

[1] Cappiello M, et al. Kinetics of human thrombin inhibition by two novel peptide inhibitors (Hirunorm IV and Hirunorm V). Biochem Pharmacol. 1996 Oct 25;52(8):1141-6. DOI:10.1016/0006-2952(96)00388-7

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