6-TAMRA CADAVERINE Chemical Properties

solubility 

DMSO, DMF, MeOH

6-TAMRA CADAVERINE Usage And Synthesis

Description

5-TAMRA cadaverine can used to modify carboxylic acid group in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be reversibly coupled to aldehydes and ketones to form a Schiff base – which can be reduced to a generate stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3).Although the mixed isomers of 5(6)-TAMRA cadaverine is a preferred, routinely used orange-fluorescent dye for staining proteins, it is rearly used for labeling peptides and nucleotides. Purification of 5(6)-TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes.

Chemical Reactivity

Primary amines

Abs/Em Maxima

553/575 nm

Extinction Coefficient

89,000 cm-1M-1

Spectrally Similar Dyes

Alexa Fluor® 546, TAMRA, CF™ 543, MB™ 543

6-TAMRA CADAVERINE()Related Product Information

• 6-TAMRA