Basic information Safety Supplier Related

NS 3694

Basic information Safety Supplier Related

NS 3694 Basic information

Product Name:
NS 3694
Synonyms:
  • Apoptosis Inhibitor II, NS3694 - CAS 426834-38-0 - Calbiochem
  • Apoptosis Inhibitor II, NS3694
  • Benzoic acid, 4-chloro-2-[[[[3-(trifluoromethyl)phenyl]amino]carbonyl]amino]-
  • NS 3694,Inhibitor,Apaf-1,Apoptosis,inhibit,caspase-9,NS-3694,apoptosome inhibitor,NS3694,cytochrome c
CAS:
426834-38-0
MF:
C15H10ClF3N2O3
MW:
358.7
Product Categories:
  • Apoptosis Inhibitors
Mol File:
426834-38-0.mol
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NS 3694 Chemical Properties

storage temp. 
2-8°C
solubility 
DMSO: 22 mg/mL, soluble
form 
solid
color 
Off-white to light yellow
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Safety Information

WGK Germany 
3
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NS 3694 Usage And Synthesis

Description

Cytochrome c, which leaks from the mitochondrial intermembrane space into the cytosol, causes oligomerization of apoptotic protease-activating factor 1 (Apaf-1), forming a caspase activation complex known as the apoptosome. NS3694 is a cell-permeable diarylurea compound that inhibits apoptosome formation and caspase activation. It blocks cytochrome c-induced caspase activation in HeLa cell cytosolic extracts with an IC50 value of approximately 50 μM. NS3694 prevents the formation of apoptosomes, which prevents the association of procaspases with Apaf-1. It suppresses caspase activation and apoptosis in cells treated with either TNF-α or staurosporine . NS3694 is used to examine the role of apoptosome-mediated caspase activation in cell death.

Uses

NS3694 is a suppressor of the formation of apoptosome Apaf-1.

Definition

ChEBI: 4-chloro-2-[[oxo-[3-(trifluoromethyl)anilino]methyl]amino]benzoic acid is a member of ureas.

in vitro

previous study found that ns3694, and its two analogs (ns1764 and ns1784) were well-tolerated by mcf-7s1 breast cancer cells at concentrations up to 100, 50, and 25 μm, respectively. moreove, all three compounds could not inhibit recombinant caspase 9 and caspase 3 at concentrations ranging from 25 to 100 μm. in addition, ns3694 was able to inhibit the co-immunoprecipitation of caspase 9 and apaf-1 from hela cell cytosol stimulated by cytochrome c and datp. ns3694 could also inhibit the formation of the active 700-kda apoptosome complex, but had no effect on tnf-induced caspase-independent death of wehi-s cells. ns3694 did not inhibit fasl-induced caspase activation or death in type i cells neither [1].

IC 50

approximately 50 μm for cytochrome c-induced caspase activation in hela cell cytosolic extracts

References

[1] lademann, u. ,cain, k.,gyrd-hansen, m., et al. diarylurea compounds inhibit caspase activation by preventing the formation of the active 700-kilodalton apoptosome complex. mol.cell biol. 23(21), 7829-7837 (2003).

NS 3694Supplier

VDM Biochemicals
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0330-2528181
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Shanghai EFE Biological Technology Co., Ltd.
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021-65675885 18964387627
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TargetMol Chemicals Inc.
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Career Henan Chemica Co
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+86-0371-86658258 +8613203830695
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ChemeGen(Shanghai) Biotechnology Co.,Ltd.
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18818260767
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