EF-5 Chemical Properties

Density 

1.66±0.1 g/cm3(Predicted)

storage temp. 

Store at -20°C

solubility 

DMSO : 125 mg/mL (413.69 mM)

form 

Solid

pka

12.32±0.46(Predicted)

color 

White to off-white

EF-5 Usage And Synthesis

Biological Activity

EF5 is a 2-nitroimidazole-based hypoxia marker corresponding to a pentafluorinated derivative of the hypoxic cell-radiation sensitizer etanidazole. Under low oxygen (hypoxic) condition, reductive activation of its 2-nitroimidazole moiety leads to enhanced EF5 adduct formation with protein thiols and reduced glutathione. The pentafluorination derivatization substantially decreases the proportion of low molecular weight hydrolytic fragmentation of the adducts, making EF5 superior to etanidazole when utilized as a hypoxia marker by immunohistological staining.