2',7'-DIFLUOROFLUORESCEIN Chemical Properties

Boiling point:

591.7±50.0 °C(Predicted)

Density 

1.70±0.1 g/cm3(Predicted)

solubility 

Insoluble in water; soluble in N,Ndimethylformamide

form 

A solid

pka

4.8, (at 22℃);4.7, (at 22℃)

color 

Orange powder

Appearance

Solid Powder

λmax

490 nm (Buffer pH 9.0)

Major Application

As geothermal tracers;TiO2 nanoparticles for light energy conversion

Biological Applications

Detecting atherosclerosis;detecting/monitoring amyloid Aβ production/accumulation/aggregation/disaggregation; diagnosing Alzheimer’s disease;estimating/measuring concentration of cortisol, melatonin, secretory IgA in body fluids;11 evaluating cetuximab-based imaging probe to target epidermal growth factor receptor (EGFR);examining biodistribution of proteins;investigating/measuring protein-protein and protein-nucleic acid interactions;measuring pH of apoplast at the root surface;visualizing hair follicles;as a substrate for measuring β-lactamase activity,phosphatases activity

InChI

InChI=1S/C20H10F2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

InChIKey

FJXJIUHGLVUXQP-UHFFFAOYSA-N

SMILES

C12(C3=C(C=C(O)C(F)=C3)OC3=C1C=C(F)C(O)=C3)C1=C(C=CC=C1)C(=O)O2

2',7'-DIFLUOROFLUORESCEIN Usage And Synthesis

Description

2',7'-difluorofluorescein is used to create bioconjugates with peptides, proteins (especially antibodies), nucleotides, oligonucleotides, drugs, hormones, lipids, and other biomolecules.

Uses

2'',7''-Difluorofluorescein is a reactant used in the synthesis of biarsenical probes that inhibit protein tyrosine phosphatases.