RHODAMINE B HYDRAZIDE
RHODAMINE B HYDRAZIDE Basic information
- Product Name:
- RHODAMINE B HYDRAZIDE
- Synonyms:
-
- RHODAMINE B HYDRAZIDE
- Spiro[1H-isoindole-1,9'-[9H]xanthen]-3(2H)-one, 2-aMino-3',6'-bis(diethylaMino)-
- RhodaMine B Cu2+ sensor
- 2-amino-3',6'-bis(diethylamino)spiro[isoindole-3,9'-xanthene]-1-one
- 2-AMINO-3',6'-BIS(DIETHYLAMINO)SPIRO[ISOINDOLE-1,9'-XANTHENE]-3-ONE
- 2-Amino-3',6'-bis(diethylamino)spiro[isoindoline-1,9'-xanthen]-3-one
- CAS:
- 74317-53-6
- MF:
- C28H32N4O2
- MW:
- 456.58
- Mol File:
- 74317-53-6.mol
RHODAMINE B HYDRAZIDE Chemical Properties
- Melting point:
- 176-177 °C
- Boiling point:
- 643.3±65.0 °C(Predicted)
- Density
- 1.27±0.1 g/cm3(Predicted)
- solubility
- DMF: 30 mg/ml; DMF:PBS(pH7.2) (1:2): 0.33 mg/ml; DMSO: 20 mg/ml; Ethanol: 0.1 mg/ml
- form
- A crystalline solid
- pka
- 6.44±0.20(Predicted)
- color
- Off-white to yellow
Safety Information
- Hazard Codes
- Xn
- Risk Statements
- 22-37/38-41
- Safety Statements
- 26-36
- WGK Germany
- 3
- HS Code
- 32049010
MSDS
- Language:English Provider:SigmaAldrich
RHODAMINE B HYDRAZIDE Usage And Synthesis
Uses
Rhodamine B hydrazide is a good probe for sulfite, with colorless and non-fluorescent properties. While the emission is related to the concentration of sulfite (5-800 ng/mL; detection limit=1.4 ng/mL (3σ)). Sulfite reduces dissolved oxygen to yield superoxide radicals, which binds to Rhodamine B hydrazide to form Rhodamine B. Rhodamine B hydrazide gives Rhodamine B-like fluorescence in the presence of sulfite, which is enhanced by Tween 80 surfactant micelles. Rhodamine B hydrazide has an absorption maximum at 554 nm and a fluorescence emission maximum at 574 nm[1].
References
[1] Yang X F, et al. Novel spectrofluorimetric method for the determination of sulfite with rhodamine B hydrazide in a micellar medium[J]. Analytica Chimica Acta, 2002, 456(1): 121-128.
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