Ani9 Chemical Properties

Density 

1.20±0.1 g/cm3(Predicted)

storage temp. 

2-8°C

solubility 

DMSO: 25 mg/ml

form 

powder

pka

11.73±0.46(Predicted)

color 

white to beige

Ani9 Usage And Synthesis

Description

Ani 9 is an inhibitor of the calcium-activated chloride channel (CaCC) anoctamin 1 (ANO1) that blocks ATP-induced apical membrane chloride currents in ANO1-expressing Fischer rat thyroid (FRT) cells (IC₅₀ = 77 nM). It is selective for ANO1 over ANO2 at 10 μM and the cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC) at 30 μM. Ani 9 inhibits ATP-induced quenching of a fluorescent intracellular halide sensor in PC3 prostate cancer cells and Capan-1 pancreatic carcinoma cells in a concentration-dependent manner.

Uses

Ani9 has been used to find out the inappropriate modulation of the membrane protein functions that are involved in the 198 ADGRG2- or Gq-mediated regulation of fluid reabsorption in the efferent ductules.

Uses

(4-Chloro-2-methylphenoxy)-[(2-methoxyphenyl)methylene]hydrazide Acetic Acid inhibits anoctamin1/transmembrane protein 16A, a calcium-activated chloride channel involved in fluid secretion, smooth muscle contraction, nociception and cancer progression.

Biochem/physiol Actions

2-(4-chloro-2-methylphenoxy)-N-[(2-methoxyphenyl)methylideneamino]-acetamide (Ani9), the most potent inhibitor, is the structural analog of Ani7. It may be considered as a novel candidate for drug therapy of cancer, hypertension, pain, diarrhea and asthma.

storage

Store at -20°C