PENTAFLUOROBENZENESULFONYL FLUORESCEIN Chemical Properties

storage temp. 

Store at -20°C

solubility 

DMF: 30 mg/ml; DMSO: 30 mg/ml; DMSO:PBS (pH 7.2)(1:1): .25 mg/ml; Ethanol: 20 mg/ml

form 

A crystalline solid

color 

Light yellow to yellow

Appearance

Solid Powder

PENTAFLUOROBENZENESULFONYL FLUORESCEIN Usage And Synthesis

Description

Reactive oxygen species (ROS) play important roles in the initiation and progression of many disease processes. Most fluorescent probes for ROS detection, such as 2,7-dichlorofluorescein (DCFH), function by an oxidative mechanism and are useful for total oxidant detection but are not selective for hydrogen peroxide specifically.¹ Pentafluorobenzenesulfonyl fluorescein is a fluorescent probe that functions by a nonoxidative mechanism. It fluoresces upon perhydrolysis of the sulfonyl linkage, and is selective for hydrogen peroxide over hydroxyl radical, tert-butyl hydroperoxide, superoxide anion, singlet oxygen, and nitrates. Pentafluorobenzenesulfonyl fluorescein can be used as a probe for hydrogen peroxide in whole cell systems.¹

Uses

Pentafluorobenzenesulfonyl fluorescein is a H2O2-selective sensor that can be used to detect H2O2 levels in cells. Pentafluorobenzenesulfonyl fluorescein is normally non-fluorescent but fluoresces upon perhydrolysis of the sulfonyl linkage by H2O2[1].

References

1. Maeda, H., Fukuyasu, Y., Yoshida, S., et al. Fluorescent probes for hydrogen peroxide based on a non-oxidative mechanism Angew. Chem. Int. Ed. Engl. 43(18),2389-2391(2004).

Excitation / Emission Maximum

465-505 nm/505-555 nm

PENTAFLUOROBENZENESULFONYL FLUORESCEIN(728912-45-6)Related Product Information

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