N-Biotinylaminoethyl Methanethiosulfonate Chemical Properties

Melting point:

95-103°C

storage temp. 

-20°C Freezer, Under Inert Atmosphere

solubility 

DMF: 30 mg/ml; DMSO: 30 mg/ml; DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml

form 

A crystalline solid

Stability:

Moisture Sensitive

InChI

InChI=1S/C13H23N3O4S3/c1-23(19,20)22-7-6-14-11(17)5-3-2-4-10-12-9(8-21-10)15-13(18)16-12/h9-10,12H,2-8H2,1H3,(H,14,17)(H2,15,16,18)/t9-,10-,12-/m0/s1

InChIKey

CQPWMXNDGWWWHG-NHCYSSNCSA-N

SMILES

CS(SCCNC(=O)CCCC[C@H]1[C@@]2([H])NC(=O)N[C@@]2([H])CS1)(=O)=O

Safety Information

HS Code 

2934999090

N-Biotinylaminoethyl Methanethiosulfonate Usage And Synthesis

Description

MTSEA-biotin is a thiol-reactive probe that can be used to label proteins at cysteine residues.

Chemical Properties

White Fluffy Solid

Uses

Reacts specifically and rapidly with thiols to form mixed disulfides. N-Biotinylaminoethyl Methanethiosulfonate can be used to probe the structures of the ACh receptor channel of the GABA receptor channel and of lactose permease. MTSEA-biotin has recently been used to probe the topology of the serotonin (5-HT) transporter by site-directed chemical labelling.

References

[1] D. WAGNER C C. Structure and Dynamics of the GABA Binding Pocket: A Narrowing Cleft that Constricts during Activation[J]. The Journal of Neuroscience, 2001, 76 1: 67-74. DOI: 10.1523/jneurosci.21-01-00067.2001
[2] MING ZHONG. Vitamin A transport and the transmembrane pore in the cell-surface receptor for plasma retinol binding protein.[J]. PLoS ONE, 2013: e73838. DOI: 10.1371/journal.pone.0073838

N-Biotinylaminoethyl Methanethiosulfonate(162758-04-5)Related Product Information

• (N-DANSYL)BIOCYTINAMIDOETHYL METHANETHIOSULFONATE
• ETHYL METHANETHIOSULFONATE
• N-Biotinylaminoethyl Methanethiosulfonate