6,8-DIFLUORO-7-HYDROXY-4-METHYLCOUMARIN Chemical Properties

Melting point:

213-215°C

Boiling point:

332.3±42.0 °C(Predicted)

Density 

1.494±0.06 g/cm3(Predicted)

storage temp. 

Hygroscopic, -20°C Freezer, Under inert atmosphere

solubility 

Insoluble in water; soluble in chloroform, N,N-dimethylformamide, dimethyl sulfoxide

pka

4.7, (at 22℃) (calcd.) 5.50 ± 0.20, most acidic, temperature: 25 °C

form 

Off-White to Pale Yellow Soild

color 

Colorless needles; white solid

λmax

358 nm (Buffers pH 10.0, pH 9.0)

Stability:

Hygroscopic

Major Application

Detecting/quantifying/measuring metals (beryllium, chromium, lead)

Biological Applications

Amplifying/detectingnucleic acid sequences; analyzing peptides, sugarchains; detecting bacteria; detecting/quantifyingnucleic acids; a substrate for measuring acylprotein thioesterase (APT) activity,, α-amylaseactivity, aryl sulfatases activity, β-galactosidasesactivity,,, guanidinobenzoatase activity, β-lactamaseactivity, lipase activity, phosphatases activity,,phospholipase LYPLAL activity, protein kinasesactivity, xylanase activity; diagnosing/treatingtraumatic brain injury; treating amyloidogenic disease;as temperature sensor,

6,8-DIFLUORO-7-HYDROXY-4-METHYLCOUMARIN Usage And Synthesis

Uses

6,8-Difluoro-7-hydroxy-4-methylcoumarin (DIFMU) was used for the diagnostic detection of natural killer cell-activity.

Definition

ChEBI: 6,8-difluoro-7-hydroxy-4-methylcoumarin is a hydroxycoumarin. It has a role as a fluorochrome.