Cholesterol probe for Smoothened Usage And Synthesis

Uses

Chol-N3 is a bioorthogonal-based chol probe. Chol-N3 can combine with super-resolution fluorescence microscopy (SRM), providing direct visualization of nanoscale lipid heterogeneity in the cell surface of resting living cells[1]. Chol-N3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

References

[1] Lorizate M, et, al. Super-Resolution Microscopy Using a Bioorthogonal-Based Cholesterol Probe Provides Unprecedented Capabilities for Imaging Nanoscale Lipid Heterogeneity in Living Cells. Small Methods. 2021 Sep;5(9):e2100430. DOI:10.1002/smtd.202100430