2-HEXADECANOYLTHIO-1-ETHYLPHOSPHORYLCHOLINE Chemical Properties

storage temp. 

Store at -20°C

solubility 

Ethanol: 30 mg/ml; PBS (pH 7.2): 5 mg/ml

2-HEXADECANOYLTHIO-1-ETHYLPHOSPHORYLCHOLINE Usage And Synthesis

Description

Thioester analogs of glycerophospholipids, in combination with Ellman’s reagent, are convenient colorimetric substrates for the measurement of phospholipase activity. HEPC is a truncated analog of phosphatidylcholine with the entire sn-1 substituent deleted, and with a hexadecylthioester in the sn-2 position.¹ HEPC is a substrate for Type II phospholipase A₂ (PLA₂) enzymes, such as porcine pancreatic, bee venom, and snake venom PLA₂. The intrinsic biological activity of HEPC has not been actively investigated.

Definition

ChEBI: 2-hexadecanoylthio-1-ethylphosphorylcholine is a member of phosphocholines.

References

1. Bhat, M.K., Mueller-Harvey, I., Sumner, I.G., et al. Simplified methods for the synthesis of 2-hexadecanoylthio-1-ethylphosphorylcholine and for the determination of phospholipase A₂ activity Biochim. Biophys. Acta 1166,244-250(1993).

2-HEXADECANOYLTHIO-1-ETHYLPHOSPHORYLCHOLINE(60793-01-3)Related Product Information

• 2-HEXADECANOYLTHIO-1-ETHYLPHOSPHORYLCHOLINE
• 1-palmitoyl-2-thiopalmitoyl phosphatidylcholine