BO-PRO 1 Chemical Properties

solubility 

Soluble in dimethyl sulfoxide

form 

Solid

Appearance

yellow solution

λmax

462 nm (H2O/DNA)

ex/em

462/481 nm

ε(extinction coefficient)

58100 cm−1M−1

Φ(quantum yield)

0.16

Biological Applications

Detecting nucleic acids, cells, microorganisms, classifying/ counting megakaryocyte; counting erythroblasts, sperms; nucleic acids amplification; nucleic acid sequencing; as temperature sensor

BO-PRO 1 Usage And Synthesis

Description

BO-TAP-1 (also known as BO-PRO^®-1) is a cyan fluorescent carbocyanine monomeric dye. BO-TAP-1 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a multiple fluorescence enhancement upon binding to dsDNA.

The bright fluorescence signal and low background make BO-TAP-1 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments.

The dye is used to detect and count cells (megakaryocyte, erythroblasts, sperms, microorganisms, etc.) and bacteria, perform nucleic acid detection, sequencing, and amplification and as a temperature sensor.

Chemical Properties

ex/em : 462/481 nm
Extinction coefficient (ε) : 58100 cm??1M??1
Quantum yield (Φ) : 0.16

Abs/Em Maxima

454 (complex)/487 (complex) nm

BO-PRO 1(157199-57-0)Related Product Information

• BO-PRO 1
• 2-([1-(3-[[3-(DIMETHYL(3-[4-[(3-METHYL-1,3-BENZOTHIAZOL-3-IUM-2-YL)METHYLENE]-1(4H)-PYRIDINYL]PROPYL)AMMONIO)PROPYL](DIMETHYL)AMMONIO]PROPYL)-4(1H)-PYRIDINYLIDENE]METHYL)-3-METHYL-1,3-BENZOTHIAZOL-3-IUM TETRAIODIDE
• 3-METHYL-2-((E)-3-[1-[3-(TRIMETHYLAMMONIO)PROPYL]-4(1H)-PYRIDINYLIDENE]-1-PROPENYL)-1,3-BENZOTHIAZOL-3-IUM DIIODIDE