4-METHYLUMBELLIFERYL OLEATE Chemical Properties

Melting point:

37-38 °C (lit.)

Boiling point:

471.81°C (rough estimate)

Density 

1.023

refractive index 

1.4840 (estimate)

Flash point:

281.3℃

storage temp. 

-20°C

solubility 

2-methoxyethanol: soluble

form 

Solid

color 

Colorless to Pale Yellow Oil

Appearance

Solid Powder

BRN 

4887028

CAS DataBase Reference

18323-58-5(CAS DataBase Reference)

Safety Information

WGK Germany 

3

F 

8-10

HS Code 

29322090

MSDS

• Language:English Provider:SigmaAldrich

4-METHYLUMBELLIFERYL OLEATE Usage And Synthesis

Description

4-Methylumbelliferyl (4-MU) oleate is a fluorogenic substrate for acid and alkaline lipases. It is cleaved by lipases, liberating 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.

Chemical Properties

Colourless Oil

Uses

Fluorogenic substrate for lipases.

Uses

4-Methylumbelliferyl oleate has been used:

• as a substrate to assay the activity of non-specific esterases
• to determine lipase activity
• as a substrate to evaluate pancreatic lipase inhibitory activity

Uses

4-Methylumbelliferyl oleate can act as Fatty acyl esters for use as fluorogenic substrates to continuously follow the hydrolytic activity of carboxylesterases, such as acid and alkaline lipases.

References

[1] M L ARMSTRONG. Lipid metabolism in xanthomatous skin of hypercholesterolemic rabbits.[J]. American Journal of Pathology, 1986, 125 2: 339-348.
[2] BERNARD J MONCLA K M P. Oleate lipase activity in Gardnerella vaginalis and reconsideration of existing biotype schemes.[J]. ACS Applied Energy Materials, 2009: 78. DOI: 10.1186/1471-2180-9-78
[3] AKIHIRO OGAWA. Lactobacillus gasseri SBT2055 suppresses fatty acid release through enlargement of fat emulsion size in vitro and promotes fecal fat excretion in healthy Japanese subjects.[J]. Lipids in Health and Disease, 2015, 14: 20. DOI: 10.1186/s12944-015-0019-0

Excitation / Emission Maximum

330 nm (pH 4.6), 370 nm (pH 7.4), 385 nm (pH 10.4)/445-454 nm

4-METHYLUMBELLIFERYL OLEATE(18323-58-5)Related Product Information

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• UMBELLIFERYL OLEATE
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