4-(4-(DIHEXADECYLAMINO)STYRYL)-N-METHYLPYRIDINIUM IODIDE Chemical Properties

storage temp. 

-20°C

solubility 

DMSO

form 

Solid

color 

Brown to reddish brown

Appearance

red-brown solid

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36

4-(4-(DIHEXADECYLAMINO)STYRYL)-N-METHYLPYRIDINIUM IODIDE Usage And Synthesis

Description

DiA is a dialkylaminostyryl dye that fluoresces when inserted into membranes or diluted into organic solvents. DiA’s excitation max is 492 nm, and emission max is 607 nm. Due to its broad emission spectrum, DiA can be detected in the green, orange, and red channels depending on the filter used.

DiA is widely used as an anterograde and retrograde neuronal tracer in living and fixed tissues and cells. DiA uniformly labels neurons via diffusion in the plasma membrane. In intact tissue, the dye does not transfer from labeled to unlabeled cells but some transfer may occur when the membrane is disrupted, for example after sectioning.

DiA can be used as a second-color neuronal tracer in conjunction with other neuronal tracers, for example, DiI.

Uses

4-Di-16-ASP is a green fluorescent membrane dye. 4-Di-16-ASP is a lipophilic aminostyryl probe with a broad emission spectrum (can be detected with green, orange or even red filters). It is commonly used for neuronal membrane tracing (it diffuses faster than DiO)[1].

Definition

ChEBI: 4-(4-dihexadecylaminostyryl)-N-methylpyridium iodide is an organic iodide salt and a pyridinium salt. It has a role as a fluorochrome. It contains a 4-(4-dihexadecylaminostyryl)-N-methylpyridium.

References

[1] Lucas McDonald,?et al. An NIR Emitting Styryl Dye with Large Stokes Shift to Enable?co-Staining Study on Zebrafish Neuromast Hair Cells. Bioorg Chem.?2019 Aug;89:103040. DOI:10.1016/j.bioorg.2019.103040

Abs/Em Maxima

492/607 nm

Fluoresene quantum yield

0.87

Extinction Coefficient

50000

4-(4-(DIHEXADECYLAMINO)STYRYL)-N-METHYLPYRIDINIUM IODIDE(114041-00-8)Related Product Information

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