Cy3 Methyltetrazine Chemical Properties

solubility 

Water, DMSO, DMF, MeOH

Appearance

Red solid

Cy3 Methyltetrazine Usage And Synthesis

Description

Methyltetrazine-activated Cy3 probe that reacts with TCO-containing compounds via an Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bioorthogonal reaction that possesses exceptional kinetics (k > 800 M-1 -1) and selectivity. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date. described to date.Cy®3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.

Uses

Trisulfo-Cy3 Methyltetrazine is a click chemistry reagent containing an methyltetrazine group. Methyltetrazine-activated Cy3 probe reacts with TCO-containing compounds via an Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures[1][2].

References

[1] Cy3 Methyltetrazine.
[2] Vera F C Ferreira, et al. In Vivo Pretargeting Based on Cysteine-Selective Antibody Modification with IEDDA Bioorthogonal Handles for Click Chemistry. Bioconjug Chem. 2021 Jan 20;32(1):121-132.

Abs/Em Maxima

553/569 nm

Extinction Coefficient

150,000

Microscopy Laser Line

532 or 555 nm

Flow Cytometry Laser Line

532 or 555 nm

Spectrally Similar Dyes

Alexa Fluor® 555, Atto™ 555, CF™ 555 Dye, DyLight™549