Cyanine 3 Tyramide Chemical Properties

solubility 

Soluble to 16 mM in DMSO

form 

Solid

color 

Brown to black

InChIKey

JPXIOSIRIWBDIC-UHFFFAOYSA-N

SMILES

C([N+]1=C(C(C)(C)C2C=C(S([O-])(=O)=O)C=CC1=2)C=CC=C1C(C2C=C(S(O)(=O)=O)C=CC=2N1CC)(C)C)CCCCC(=O)NCCC1C=CC(O)=CC=1

Cyanine 3 Tyramide Usage And Synthesis

Uses

Cyanine 3 Tyramide (Tyramide-Cy3), an orange fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids[1][2].

storage

Store at -20°C

References

[1] Chao J, et, al. Immunofluorescence signal amplification by the enzyme-catalyzed deposition of a fluorescent reporter substrate (CARD). Cytometry. 1996 Jan 1;23(1):48-53. DOI:10.1002/(SICI)1097-0320(19960101)23:13.0.CO;2-I
[2] Schmidt BF, et, al. Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide. J Histochem Cytochem. 1997 Mar;45(3):365-73. DOI:10.1177/002215549704500304