AF430 NHS ester Chemical Properties

solubility 

Soluble in water, DMSO, DMF, etc.

Appearance

Yellow solid

ex/em

430/545 nm

ε(extinction coefficient)

15,000 L⋅mol−1⋅cm−1

Φ(quantum yield)

0.23

AF430 NHS ester Usage And Synthesis

Description

AF 430 is a hydrophilic dye. It is used in flow cytometry and other applications.

The NHS ester functional group allows to conjugate the fluorophore efficiently with primary and secondary amine groups in both aqueous buffers and anhydrous conditions.

Chemical Properties

Appearance: Yellow solid
ex/em: 430/545 nm
Extinction coefficient (ε): 15,000 L??mol??1??cm??1
Quantum yield (Φ): 0.23

Uses

AF430 NHS ester is an AF 430 maleimide is a derivative of the yellow fluorescent dye AF 430. AF430 has an excitation wavelength of 425 nm and an emission wavelength of 542 nm. AF430 NHS ester can be uesd for the labeling of amino-groups in peptides, proteins, and oligonucleotides. To achieve specific coupling of dye labels and biomolecules[1].

Definition

ChEBI: Alexa Fluor 430 is a methanesulfonate salt, a pyrrolidinone and an organic heterotricyclic compound. It has a role as a fluorochrome. It contains a triethylammonium ion and an Alexa Fluor 430(1-).

References

[1] Fernández B, Gálvez N, Sánchez P, et al. Fluorescence resonance energy transfer in ferritin labeled with multiple fluorescent dyes[J]. JBIC Journal of Biological Inorganic Chemistry, 2008, 13: 349-355.

Abs/Em Maxima

430/542 nm

Fluoresene quantum yield

0.23

Extinction Coefficient

15955

CF260

0,59

CF280

0,24

AF430 NHS ester(467233-94-9)Related Product Information

• 2H-Pyrano[3,2-g]quinoline-9(8H)-hexanoic acid, 8,8-dimethyl-2-oxo-6-(sulfomethyl)-4-(trifluoromethyl)-, sodium salt (1:1)
• Alexa Fluor 430 Acid